ArticleProceedings of the Japan Academy. Series B, Physical and biological sciences2020
Heterologous production of fungal natural products: Reconstitution of biosynthetic gene clusters in model host Aspergillus oryzae.
Article in Proceedings of the Japan Academy. Series B, Physical and biological sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 16 citations in OpenAlex.
- Black yeasts emerge as hosts for fungal polyketide production.Nature chemical biology · 2026Article
- CRISPR-Cas9 Gene Editing inBiology · 2025Review
- Recent Advances in Heterologous Protein Expression and Natural Product Synthesis byJournal of fungi (Basel, Switzerland) · 2025Review
- Genetically-modified activation strategy facilitates the discovery of sesquiterpene-derived metabolites fromSynthetic and systems biotechnology · 2025Article
- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Potential Bioactivities, Chemical Composition, and Conformation Studies of Exopolysaccharide-DerivedJournal of fungi (Basel, Switzerland) · 2024Article
- Exploration of diverse secondary metabolites from Penicillium brasilianum by co-culturing with Armillaria mellea.Applied microbiology and biotechnology · 2024Article
- Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation inJournal of fungi (Basel, Switzerland) · 2024Review
- Synthetic Biology Tools for EngineeringJournal of fungi (Basel, Switzerland) · 2024Review
- Identification and Characterization of an Antifungal GeneInternational journal of molecular sciences · 2023Article
- Application of Gene Knockout and Heterologous Expression Strategy in Fungal Secondary Metabolites Biosynthesis.Marine drugs · 2022Review
- CRISPR/Cas9-Based Genome Editing and Its Application inJournal of fungi (Basel, Switzerland) · 2022Review
- Reconstitution of biosynthetic pathway for mushroom-derived cyathane diterpenes in yeast and generation of new "non-natural" analogues.Acta pharmaceutica Sinica. B · 2021Article
- Advances in Genetic Engineering Technology and Its Application in the Industrial FungusFrontiers in microbiology · 2021Review
- Effects of Nitrogen and Phosphorus Limitation on Fatty Acid Contents inFrontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
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Abstract
While exploring phytotoxic metabolites from phytopathogenic fungi in the 1970s, we became interested in biosynthetic enzymes that catalyze Diels-Alder reactions involving biosynthesis of several phytotoxins that we isolated. Target enzymes were successfully characterized, and this triggered the identification of various Diels-Alderases in a recent decade. Through our Diels-Alderase project in 1990s, we recognized a highly efficient expression system of various biosynthetic genes with Aspergillus oryzae as a host. With the development of tools such as genomic data and bioinformatics analysis to identify biosynthetic gene clusters for natural products, we developed a highly reliable methodology such as hot spot knock-in to elucidate the biosynthetic pathways of representative fungal metabolites including phytotoxic substances. This methodology allows total biosynthesis of natural products and genome mining using silent biosynthetic gene clusters to obtain novel bioactive metabolites. Further applications of this technology are discussed.
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