ArticleBMC plant biology2020
Simultaneous induction of mutant alleles of two allergenic genes in soybean by using site-directed mutagenesis.
Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Targeted Reduction of Specific Antinutritional and Allergen-Associated Proteins to Advance Soybean Seed Quality.International journal of molecular sciences · 2026Article
- CRISPR/Cas9 ribonucleoprotein-mediated knockout ofFrontiers in plant science · 2026Article
- CRISPR/Cas9-Targeted Gene Editing of Allergenic Profilin-EncodingPlants (Basel, Switzerland) · 2025Article
- CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.Journal of advanced research · 2025Review
- Modulation of Root Hydrotropism and Recovery From Drought by MIZ1-like Genes in Tomato.Plant, cell & environment · 2025Article
- Genome editing, an opportunity to revive soybean cultivation in Europe.The Plant journal : for cell and molecular biology · 2025Review
- Simultaneous site-directed mutagenesis for soybean ß-amyrin synthase genes via DNA-free CRISPR/Cas9 system using a single gRNA.Plant cell reports · 2025Article
- Off-target sequence variations driven by the intrinsic properties of the Cas-sgRNA-DNA complex in genome editing.PloS one · 2025Article
- Editorial: Engineering future crops through genome editing.Frontiers in plant science · 2025Article
- CRISPR/Cas9-mediated simultaneous targeting of GmP34 and its homologs produces T-DNA-free soybean mutants with reduced allergenic potential.Frontiers in plant science · 2025Article
- Gene editing in allergic diseases: Identification of novel pathways and impact of deleting allergen genes.The Journal of allergy and clinical immunology · 2024Review
- Comparative Evaluation of Transient Protein Expression Efficiency in Tissues across Soybean Varieties Using the Tsukuba System.Plants (Basel, Switzerland) · 2024Article
- Precision engineering for localization, validation, and modification of allergenic epitopes.The Journal of allergy and clinical immunology · 2024Review
- A review and outlook on expression of animal proteins in plants.Frontiers in plant science · 2024Review
- Soybean genetics, genomics, and breeding for improving nutritional value and reducing antinutritional traits in food and feed.The plant genome · 2023Review
- Prospects for developing allergen-depleted food crops.The plant genome · 2023Review
- Recent advances in the improvement of soybean seed traits by genome editing.Plant biotechnology (Tokyo, Japan) · 2023Article
- Site-directed mutagenesis of soybeanPlant biotechnology (Tokyo, Japan) · 2023Article
- Bioinformatic and literature assessment of toxicity and allergenicity of a CRISPR-Cas9 engineered gene drive to control Anopheles gambiae the mosquito vector of human malaria.Malaria journal · 2023Article
- Foreign DNA detection in genome-edited potatoes by high-throughput sequencing.Scientific reports · 2023Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSoybean (Glycine max) is a major protein crop, because soybean protein has an amino acid score comparable to that of beef and egg white. However, many allergens have been identified among soybean proteins. A decrease in allergenic protein levels would be useful for expanding the market for soybean proteins and processed foods. Recently, the CRISPR/Cas9 system has been adopted as a powerful tool for the site-directed mutagenesis in higher plants. This system is expected to generate hypoallergenic soybean varieties.
resultsWe used two guide RNAs (gRNAs) and Agrobacterium-mediated transformation for simultaneous site-directed mutagenesis of two genes encoding the major allergens Gly m Bd 28 K and Gly m Bd 30 K in two Japanese soybean varieties, Enrei and Kariyutaka. We obtained two independent T
conclusionsSimultaneous site-directed mutagenesis by the CRISPR/Cas9 system removed two major allergenic proteins from mature soybean seeds. This system enables rapid and efficient modification of seed components in soybean varieties.
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