Evidence map›Paper›PMID 33174608›Full record

ArticleMolecular medicine reports2020

Tripartite motif‑containing 14 regulates cell proliferation and apoptosis in cervical cancer via the Akt signaling pathway.

Wenjing Diao, Caiying Zhu, Qisang Guo, Yuankui Cao, Yu Song, Hua Feng, Jun Li, Xiaohong Xue, Pei Lu

RetractedOpen access · hybridAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 14 citations in OpenAlex.

  1. Advances in the antitumor mechanisms of tripartite motif-containing protein 3.Journal of cancer research and clinical oncology · 2024
    Review
  2. Review
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Wenjing DiaoMedical Center of Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China.
Caiying ZhuMedical Center of Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China.
Qisang GuoMedical Center of Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China.
Yuankui CaoMedical Center of Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China.
Yu SongMedical Center of Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China.
Hua FengMedical Center of Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China.
Jun LiDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China.
Xiaohong XueDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China.
Pei LuDepartment of Clinical Laboratory, Shanghai No. 8 People's Hospital, Shanghai 200235, P.R. China.
Obstetrics and Gynecology Hospital of Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tripartite motif‑containing (TRIM) 14 is a protein of the TRIM family. Studies have indicated that TRIM14 may be used as an oncogene in tumor cells, such as osteosarcoma, non‑small cell lung cancer and breast cancer through different pathways. However, the functions of TRIM14 in cervical cancer cells remain unclear. Therefore, this study aimed to investigate the functions of TRIM14 in cervical cancer cells and its underlying mechanism. Caski cells stably expressing TRIM14 and SiHa, and HeLa cells stably expressing TRIM14 short hairpin RNA were constructed by lentivirus‑mediated overexpression or knockdown systems. The effects of TRIM14 on proliferation and apoptosis of cervical cancer cells were detected by Cell Counting Kit‑8 (CCK‑8) assay and flow cytometry, respectively. In addition, reverse transcription‑quantitative (RT‑q) PCR and western blotting were used to investigate the expression levels of TRIM14 and of signaling pathway marker protein including P21, caspase‑3, cleaved caspase‑3, Akt and phosphorylated Akt. The results of RT‑qPCR and western blotting revealed that TRIM14 was highly expressed in human cervical cancer tissues and cell lines compared with adjacent normal tissues and normal cervical epithelial cells. TRIM14 also regulated cell proliferation and apoptosis of human SiHa, HeLa and Caski cervical cancer cell lines through the Akt signaling pathway. Additionally, TRIM14 protein levels were related to the clinical and pathological features of cervical cancer. CCK‑8 assay and flow cytometry demonstrated that TRIM14 expression could promote cervical cancer cell proliferation and autophagy suppression. Taken together, TRIM14‑induced cell proliferation and apoptosis inhibition may by evoked by the activation of the Akt pathway. This study demonstrated the role of TRIM14 in cervical cancer, and reveals its mechanism of action as a potential therapeutic target for cervical cancer.

Indexed as

AdultAgedApoptosisCell Line, TumorCell MovementCell ProliferationCervix UteriChinaFemaleGene Expression Regulation, NeoplasticHeLa CellsHumansIntracellular Signaling Peptides and ProteinsMiddle AgedOncogenesOsteosarcomaIntracellular Signaling Peptides and ProteinsProto-Oncogene Proteins c-aktTRIM14 protein, humanTripartite Motif Proteins

Identifiers

PMID33174608
PMCPMC7646967
OpenAlexW3095077953

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.