ArticleNeurogastroenterology and motility2021
Intrinsic cholinergic innervation in the human sigmoid colon revealed using CLARITY, three-dimensional (3D) imaging, and a novel anti-human peripheral choline acetyltransferase (hpChAT) antiserum.
Article in Neurogastroenterology and motility, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 17 citations in OpenAlex.
- Altered Sigmoid Mucosal Innervation and Mast Cell Proximity to Sensory Nerve Fibers Are Associated With Symptom Severity in Patients With Irritable Bowel Syndrome.Neurogastroenterology and motility · 2025Article
- Three-dimensional imaging and computational quantitation as a novel approach to assess nerve fibers, enteric glial cells, mast cells, and the proximity of mast cells to the nerve fibers in human sigmoid mucosal biopsies from healthy subjects.Journal of neuroscience methods · 2025Article
- Recent advances in facilitating the translation of bioelectronic medicine therapies.Current opinion in biomedical engineering · 2025Article
- Imaging the enteric nervous system.Frontiers in neuroanatomy · 2025Review
- AI-powered 3D pathology protocol enhances enteric nervous system visualization and quantification for clinical diagnostics.Theranostics · 2025Article
- The parasympathetic and sensory innervation of the proximal and distal colon in male mice.Frontiers in neuroanatomy · 2024Article
- Distribution, quantification, and characterization of substance P enteric neurons in the submucosal and myenteric plexuses of the porcine colon.Cell and tissue research · 2024Article
- The enteric nervous system.Physiological reviews · 2023Review
- New insight on the enteric cholinergic innervation of the pig colon by central and peripheral nervous systems: reduction by repeated loperamide administration.Frontiers in neuroscience · 2023Article
- Vasculature in the mouse colon and spatial relationships with the enteric nervous system, glia, and immune cells.Frontiers in neuroanatomy · 2023Article
- Whole gut imaging allows quantification of all enteric neurons in the adult zebrafish intestine.Neurogastroenterology and motility · 2022Article
- Transduction of Systemically Administered Adeno-Associated Virus in the Colonic Enteric Nervous System and c-Kit Cells of Adult Mice.Frontiers in neuroanatomy · 2022Article
- Label-free, fast, 2-photon volume imaging of the organization of neurons and glia in the enteric nervous system.Frontiers in neuroanatomy · 2022Article
- Classification of human enteric neurons.Histochemistry and cell biology · 2021Review
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6 authors at 3 institutions in 2 countries.
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Abstract
backgroundWe previously reported the specificity of a novel anti-human peripheral choline acetyltransferase (hpChAT) antiserum for immunostaining of cholinergic neuronal cell bodies and fibers in the human colon. In this study, we investigate 3D architecture of intrinsic cholinergic innervation in the human sigmoid colon and the relationship with nitrergic neurons in the enteric plexus.
methodsWe developed a modified CLARITY tissue technique applicable for clearing human sigmoid colon specimens and immunostaining with hpChAT antiserum and co-labeling with neuronal nitric oxide synthase (nNOS) antibody. The Z-stack confocal images were processed for 3D reconstruction/segmentation/digital tracing and computational quantitation by Imaris 9.2 and 9.5. KEY
resultsIn the mucosa, a local micro-neuronal network formed of hpChAT-ir fibers and a few neuronal cell bodies were digitally assembled. Three layers of submucosal plexuses were displayed in 3D structure that were interconnected by hpChAT-ir fiber bundles and hpChAT-ir neurons were rarely co-labeled by nNOS. In the myenteric plexus, 30.1% of hpChAT-ir somas including Dogiel type I and II were co-labeled by nNOS and 3 classes of hpChAT-ir nerve fiber strands were visualized in 3D images and videos. The density and intensity values of hpChAT-ir fibers in 3D structure were significantly higher in the circular than in the longitudinal layer. CONCLUSIONS AND INFERENCES: The intrinsic cholinergic innervation in the human sigmoid colon was demonstrated layer by layer for the first time in 3D microstructures. This may open a new venue to assess the structure-function relationships and pathological alterations in colonic diseases.
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