ArticleMolecular oral microbiology2021
Modified SHI medium supports growth of a disease-state subgingival polymicrobial community in vitro.
Article in Molecular oral microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 19 citations in OpenAlex.
- Targeting of Oral Bacterial Taxa Linked to Disease by Flavonols.Nutrients · 2026Article
- Protocol for spectrophotometric determination of titanium microparticles in biological samples via Ti(IV)-HSTAR protocols · 2026Article
- Biofilm removal capacity and titanium surface integrity in non-abrasive versus abrasive peri-implantitis cleaning interventions.Journal of periodontology · 2026Article
- Nutritional pressure from serum amplifies dysbiotic features in an oral microbiome synthetic community.The ISME journal · 2026Article
- Identification of interbacterial adhesion between oral Schaalia species and streptococcal serine-rich repeat glycoproteins.FEMS microbiology letters · 2026Article
- Examining Oral Microbial Communities at the Gingival Interface During Health and Inflammatory Diseases.Advances in experimental medicine and biology · 2026Review
- Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes.NPJ biofilms and microbiomes · 2025Article
- Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols.Scientific reports · 2025Article
- The Impact of Human Salivary Amylase Gene Copy Number and Starch on Oral Biofilms.Microorganisms · 2025Article
- Utilizing a naturopathic mouthwash with selective antimicrobial effects against multispecies oral biofilms for prevention of dysbiosis.Frontiers in oral health · 2025Article
- Exploring ex vivo biofilm dynamics: consequences of low ampicillin concentrations on the human oral microbiome.NPJ biofilms and microbiomes · 2024Article
- Construction of a versatile in vitro cultivation screening platform using human oral microbiota.Environmental microbiology reports · 2024Article
- Dynamic assessment of titanium surface oxides following mechanical damage reveals only partial passivation under inflammatory conditions.Npj Materials degradation · 2024Article
- Localized microbially induced inflammation influences distant healthy tissues in the human oral cavity.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Effects of Preservation and Propagation Methodology on Microcosms Derived from the Oral Microbiome.Microorganisms · 2022Article
- Effect of Calcium Ion Supplementation on Oral Microbial Composition and Biofilm Formation In Vitro.Microorganisms · 2022Article
- Effects of the Probiotic,Animals : an open access journal from MDPI · 2022Article
- In vitro model systems for exploring oral biofilms: From single-species populations to complex multi-species communities.Journal of applied microbiology · 2022Review
- Succession of oral bacterial colonizers on dental implant materials: An in vitro biofilm model.Dental materials : official publication of the Academy of Dental Materials · 2022Article
- Complete Genomes of Clade G6mSphere · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Developing a laboratory model of oral polymicrobial communities is essential for in vitro studies of the transition from healthy to diseased oral plaque. SHI medium is an enriched growth medium capable of supporting in vitro biofilms with similar diversity to healthy supragingival inocula; however, this medium does not maintain the diversity of gram-negative bacteria more associated with subgingival plaque. Here, we systematically modified SHI medium components to investigate the impacts of varying nutrients and develop a medium capable of supporting a specific disease-state subgingival community. A diseased subgingival plaque sample was inoculated in SHI medium with increasing concentrations of sucrose (0%, 0.1%, 0.5%), fetal bovine serum (FBS) (0%, 10%, 20%, 30%, 50%), and mucin (0.1, 2.5, 8.0 g/L) and grown for 48 hrs, then the 16S rRNA profiles of the resulting biofilms were examined. In total, these conditions were able to capture 89 of the 119 species and 43 of the 51 genera found in the subgingival inoculum. Interestingly, biofilms grown in high sucrose media, although dominated by acidogenic Firmicutes with a low final pH, contained several uncultured taxa from the genus Treponema, information that may aid culturing these periodontitis-associated fastidious organisms. Biofilms grown in a modified medium (here named subSHI-v1 medium) with 0.1% sucrose and 10% FBS had a high diversity closest to the inoculum and maintained greater proportions of many gram-negative species of interest from the subgingival periodontal pocket (including members of the genera Prevotella and Treponema, and the Candidate Phyla Radiation phylum Saccharibacteria), and therefore best represented the disease community.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.