Evidence map›Paper›PMID 33173862›Full record

ArticlebioRxiv : the preprint server for biology2021

The translational landscape of SARS-CoV-2 and infected cells.

Maritza Puray-Chavez, Nakyung Lee, Kasyap Tenneti, Yiqing Wang, Hung R Vuong, Yating Liu, Amjad Horani, Tao Huang, Sean P Gunsten, James B Case and 5 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 19 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors at 1 institution in 1 country.

Maritza Puray-ChavezDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Nakyung LeeDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Kasyap TennetiDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Yiqing WangDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Hung R VuongDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Yating LiuDepartment of Genetics, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Amjad HoraniDepartment of Pediatrics, Allergy, Immunology and Pulmonary Medicine, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Tao HuangDepartment of Medicine, Pulmonary and Critical Care Medicine, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Sean P GunstenDepartment of Medicine, Pulmonary and Critical Care Medicine, Washington University School of Medicine, Saint Louis, MO 63110, USA.
James B CaseDepartment of Medicine, Infectious Disease Division, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Wei YangDepartment of Genetics, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Michael S DiamondDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Steven L BrodyDepartment of Medicine, Pulmonary and Critical Care Medicine, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Joseph DoughertyDepartment of Genetics, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Sebla B KutluayDepartment of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Washington University in St. Louis · US

Funding

Translational regulation of HIV-1 replication and host responsesR21AI145669 · NIAID · WASHINGTON UNIVERSITY · PI KUTLUAY, SEBLA B. · 2019 to 2020
$431k
NIAID NIH HHS R21 AI145669
6 · The paper itself

Abstract

SARS-CoV-2 utilizes a number of strategies to modulate viral and host mRNA translation. Here, we used ribosome profiling in SARS-CoV-2 infected model cell lines and primary airway cells grown at the air-liquid interface to gain a deeper understanding of the translationally regulated events in response to virus replication. We find that SARS-CoV-2 mRNAs dominate the cellular mRNA pool but are not more efficiently translated than cellular mRNAs. SARS-CoV-2 utilized a highly efficient ribosomal frameshifting strategy in comparison to HIV-1, suggesting utilization of distinct structural elements. In the highly permissive cell models, although SARS-CoV-2 infection induced the transcriptional upregulation of numerous chemokines, cytokines and interferon stimulated genes, many of these mRNAs were not translated efficiently. Impact of SARS-CoV-2 on host mRNA translation was more subtle in primary cells, with marked transcriptional and translational upregulation of inflammatory and innate immune responses and downregulation of processes involved in ciliated cell function. Together, these data reveal the key role of mRNA translation in SARS-CoV-2 replication and highlight unique mechanisms for therapeutic development.

Indexed as

immune responsemRNA translationribo-seqribosomal frameshiftingribosome profilingSARS-CoV-2translational repressionvirus-host interactionvirus replication

Identifiers

PMID33173862
PMCPMC7654850
OpenAlexW3096815306

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.