Evidence map›Paper›PMID 33173602›Full record

ArticleActa naturae

GLAD-PCR Assay of R(5mC)GY Sites in the Regulatory Region of Tumor-Suppressor Genes Associated with Gastric Cancer.

B S Malyshev, N A Netesova, N A Smetannikova, M A Abdurashitov, A G Akishev, E V Dubinin, A Z Azanov, I V Vihlyanov, M K Nikitin, A B Karpov and 1 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta naturae. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

B S MalyshevState Research Center of Virology and Biotechnology «Vector», Novosibirsk region, Koltsovo, 630559 Russia.
N A NetesovaState Research Center of Virology and Biotechnology «Vector», Novosibirsk region, Koltsovo, 630559 Russia.
N A SmetannikovaState Research Center of Virology and Biotechnology «Vector», Novosibirsk region, Koltsovo, 630559 Russia.
M A AbdurashitovEpiGene LLC, Novosibirsk, 630090 Russia.
A G AkishevEpiGene LLC, Novosibirsk, 630090 Russia.
E V DubininEpiGene LLC, Novosibirsk, 630090 Russia.
A Z AzanovRegional Clinical Oncology Center, Kemerovo, 650036 Russia.
I V VihlyanovAltai Regional Oncology, CenterAltai region, Barnaul, 656049 Russia.
M K NikitinAltai Regional Oncology, CenterAltai region, Barnaul, 656049 Russia.
A B KarpovSeversk Biophysical Research Centre, Tomsk region, Seversk, 636039 Russia.
S Kh DegtyarevState Research Center of Virology and Biotechnology «Vector», Novosibirsk region, Koltsovo, 630559 Russia.
Medigene (Germany) · DEState Research Center of Virology and Biotechnology VECTOR · RUAltai State University · RUKemerovo Cardiology Center · RUSeversk Technological Institute · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

At early stages of carcinogenesis, the regulatory regions of some tumor suppressor genes become aberrantly methylated at RCGY sites, which are substrates of DNA methyltransferase Dnmt3. Identification of aberrantly methylated sites in tumor DNA is considered to be the first step in the development of epigenetic PCR test systems for early diagnosis of cancer. Recently, we have developed a GLAD-PCR assay, a method for detecting the R(5mC)GY site in the genome position of interest even at significant excess of DNA molecules with a non-methylated RCGY site in this location. The aim of the present work is to use the GLAD-PCR assay to detect the aberrantly methylated R(5mC)GY sites in the regulatory regions of tumor suppressor genes (

Indexed as

DNA methylationgastric cancerGLAD-PCR assaymethyl-directed DNA endonuclease GlaItumor suppressor genes

Identifiers

PMID33173602
PMCPMC7604892
OpenAlexW3095484980

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.