Evidence map›Paper›PMID 33172891›Full record

ArticleThe Journal of biological chemistry

Discovery of an ultraspecific triuret hydrolase (TrtA) establishes the triuret biodegradation pathway.

Lambros J Tassoulas, Mikael H Elias, Lawrence P Wackett

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Lambros J TassoulasDepartment of Biochemistry, Biophysics & Molecular Biology, University of Minnesota, Minneapolis, Minnesota, USA; BioTechnology Institute, University of Minnesota, St Paul, Minnesota, USA.
Mikael H EliasDepartment of Biochemistry, Biophysics & Molecular Biology, University of Minnesota, Minneapolis, Minnesota, USA; BioTechnology Institute, University of Minnesota, St Paul, Minnesota, USA.
Lawrence P WackettDepartment of Biochemistry, Biophysics & Molecular Biology, University of Minnesota, Minneapolis, Minnesota, USA; BioTechnology Institute, University of Minnesota, St Paul, Minnesota, USA. Electronic address: wacke003@umn.edu.
University of Minnesota · US

Funding

TRAINING FOR FUTURE BIOTECHNOLOGY DEVELOPMENTT32GM008347 · NIGMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI SCHMIDT-DANNERT, CLAUDIA · 1990 to 2021
$11.0M
NIGMS NIH HHS T32 GM008347
6 · The paper itself

Abstract

Triuret (carbonyldiurea) is an impurity found in industrial urea fertilizer (<0.1% w/w) that is applied, worldwide, around 300 million pounds each year on agricultural lands. In addition to anthropogenic sources, endogenous triuret has been identified in amoeba and human urine, the latter being diagnostic for hypokalemia. The present study is the first to describe the metabolic breakdown of triuret, which funnels into biuret metabolism. We identified the gene responsible for triuret decomposition (trtA) in bacterial genomes, clustered with biuH, which encodes biuret hydrolase and has close protein sequence homology. TrtA is a member of the isochorismatase-like hydrolase (IHL) protein family, similarly to BiuH, and has a catalytic efficiency (k

Indexed as

Biodegradation, EnvironmentalCatalytic DomainCrystallography, X-RayGenome, BacterialHydrolasesHydrolysisKineticsProtein ConformationSoil MicrobiologySubstrate SpecificityUreaHydrolasesisochorismatasetriuretUreaBiuHbiuret;cysteine hydrolaseenzyme evolutionIHL protein familynitrogen-containing compoundsubstrate discriminationtriuretTrtAurea pyrolysisuric acid oxidation

Identifiers

PMID33172891
PMCPMC7948467
OpenAlexW3103382093

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.