ArticleBMC infectious diseases2020
Evaluation of TaqMan Array card (TAC) for the detection of 28 respiratory pathogens.
Article in BMC infectious diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- Broad-spectrum molecular detection of zoonotic agents in wild micromammals: a nationwide surveillance study in the Republic of Korea.Parasites & vectors · 2026Article
- Performance evaluation of TaqMan Array Card real-time PCR for multi-pathogen detection in acute undifferentiated febrile illness.BMC infectious diseases · 2026Article
- Surveillance of respiratory viruses by aerosol screening in indoor air as an early warning system for epidemics.Environmental microbiology reports · 2024Article
- Site-Specific Analysis of the Incidence Rate of Enterotoxigenic Escherichia coli Infection Elucidates an Association with Childhood Stunting, Wasting, and Being Underweight: A Secondary Analysis of the MAL-ED Birth Cohort.The American journal of tropical medicine and hygiene · 2023Article
- The rapid detection of respiratory pathogens in critically ill children.Critical care (London, England) · 2023Observational
- Side-by-side comparison of parent vs. technician-collected respiratory swabs in low-income, multilingual, urban communities in the United States.BMC public health · 2022Article
- Rapid Assay for Sick Children with Acute Lung infection Study (RASCALS): diagnostic cohort study protocol.BMJ open · 2021Article
- Occurrence of the Legionella species in the respiratory samples of patients with pneumonia symptoms from Ahvaz, Iran; first detection of Legionella cherrii.Molecular biology reports · 2021Article
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Authors and funding
10 authors at 6 institutions in 1 country.
Funding
Abstract
backgroundRespiratory infections are a serious threat to human health. So, rapid detection of all respiratory pathogens can facilitate prompt treatment and prevent the deterioration of respiratory disease. Previously published primers and probes of the TaqMan array card (TAC) for respiratory pathogens are not sensitive to Chinese clinical specimens. This study aimed to develop and improve the TAC assay to detect 28 respiratory viral and bacterial pathogens in a Chinese population.
methodsTo improve the sensitivity, we redesigned the primers and probes, and labeled the probes with minor groove binders. The amplification efficiency, sensitivity, and specificity of the primers and probes were determined using target-gene containing standard plasmids. The detection performance of the TAC was evaluated on 754 clinical specimens and the results were compared with those from conventional methods.
resultsThe performance of the TAC assay was evaluated using 754 clinical throat swab samples and the results were compared with those from gold-standard methods. The sensitivity and specificity were 95.4 and 96.6%, respectively. The lowest detection limit of the TAC was 10 to 100 copies/μL.
conclusionsTAC is an efficient, accurate, and high-throughput approach to detecting multiple respiratory pathogens simultaneously and is a promising tool for the identification of pathogen outbreaks.
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