ArticleInternational journal of molecular sciences2020
Shear Stress Modulates Osteoblast Cell and Nucleus Morphology and Volume.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Bone tissue regeneration: role of osteocyte mechanosensing and mechanotransduction.Stem cells translational medicine · 2026Review
- Mechanosensitive ion channels as novel targets in osteoporosis.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2026Review
- β2-Adrenergic receptor regulates osteoblast differentiation and migration and C-terminal β-catenin phosphorylation.Biochemical and biophysical research communications · 2025Article
- Ageing-Related Changes in Ultrastructural Bone Matrix Composition and Osteocyte Mechanosensitivity.Current osteoporosis reports · 2025Review
- Bionic Nanostructures Create Mechanical Signals to Mediate the Composite Structural Bone Regeneration Through Multi-System Regulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Mechanosignaling in Osteoporosis: When Cells Feel the Force.International journal of molecular sciences · 2025Review
- Modular cone-and-plate device for mechanofluidic assays in Transwell inserts.Frontiers in bioengineering and biotechnology · 2025Article
- Role and mechanism of Integrin α5β1 in bone formation and disease.Frontiers in cell and developmental biology · 2025Review
- Low, but Not High, Pulsating Fluid Shear Stress Affects Matrix Extracellular Phosphoglycoprotein Expression, Mainly via Integrin β Subunits in Pre-Osteoblasts.Current issues in molecular biology · 2024Article
- Identification of osteoblastic autophagy-related genes for predicting diagnostic markers in osteoarthritis.iScience · 2024Article
- Comparative Analysis of Osteoblastic Responses to Titanium and Alumina-Toughened Zirconia Implants: An In Vitro Study.Biomolecules · 2024Article
- The bone nonunion microenvironment: A place where osteogenesis struggles with osteoclastic capacity.Heliyon · 2024Review
- Mechanobiology in Cells and Tissues.International journal of molecular sciences · 2023Article
- The role of integrin family in bone metabolism and tumor bone metastasis.Cell death discovery · 2023Review
- Fluid shear stress regulates the survival of circulating tumor cells via nuclear expansion.Journal of cell science · 2022Article
- Appreciating the role of cell shape changes in the mechanobiology of epithelial tissues.Biophysics reviews · 2022Review
- Effects of Mechanical Stress Stimulation on Function and Expression Mechanism of Osteoblasts.Frontiers in bioengineering and biotechnology · 2022Review
- Aberrant Fluid Shear Stress Contributes to Articular Cartilage Pathogenesis via Epigenetic Regulation of ZBTB20 by H3K4me3.Journal of inflammation research · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Mechanical loading preserves bone mass and function-yet, little is known about the cell biological basis behind this preservation. For example, cell and nucleus morphology are critically important for cell function, but how these morphological characteristics are affected by the physiological mechanical loading of bone cells is under-investigated. This study aims to determine the effects of fluid shear stress on cell and nucleus morphology and volume of osteoblasts, and how these effects relate to changes in actin cytoskeleton and focal adhesion formation. Mouse calvaria 3T3-E1 (MC3T3-E1) osteoblasts were treated with or without 1 h pulsating fluid flow (PFF). Live-cell imaging was performed every 10 min during PFF and immediately after PFF. Cytoskeletal organization and focal adhesions were visualized, and gene and protein expression quantified. Two-dimensional (2D) and three-dimensional (3D) morphometric analyses were made using MeasureStack and medical imaging interaction toolkit (MITK) software. 2D-images revealed that 1 h PFF changed cell morphology from polygonal to triangular, and nucleus morphology from round to ellipsoid. PFF also reduced cell surface area (0.3-fold), cell volume (0.3-fold), and nucleus volume (0.2-fold). During PFF, the live-cell volume gradually decreased from 6000 to 3000 µm
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.