Evidence map›Paper›PMID 33171642›Full record

ArticleBiosensors2020

Single-Molecule FRET Detection of Sub-Nanometer Distance Changes in the Range below a 3-Nanometer Scale.

Heyjin Son, Woori Mo, Jaeil Park, Joong-Wook Lee, Sanghwa Lee

Open access · goldAbstract read
In one paragraph

Article in Biosensors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
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  4. bioRxiv : the preprint server for biology · 2024
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  9. Regulation of the DEAH/RHA helicase Prp43 by the G-patch factor Pfa1.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Heyjin SonAdvanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
Woori MoAdvanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
Jaeil ParkAdvanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.
Joong-Wook LeeDepartment of Physics and Optoelectronics Convergence Research Center, Chonnam National University, Gwangju 61186, Korea.
Sanghwa LeeAdvanced Photonics Research Institute, Gwangju Institute of Science and Technology, Gwangju 61005, Korea.ORCID 0000-0001-6986-7583
Chonnam National University · KRGwangju Institute of Science and Technology · KR

Funding

National Research Foundation of Korea 2018R1D1A1B07051186National Research Foundation of Korea 2019R1C1C1008438National Research Foundation of Korea 2020M3A9I4038197
6 · The paper itself

Abstract

Single-molecule fluorescence energy transfer (FRET) detection has become a key technique to monitor intra- and intermolecular distance changes in biological processes. As the sensitive detection range of conventional FRET pairs is limited to 3-8 nm, complement probes are necessary for extending this typical working range. Here, we realized a single-molecule FRET assay for a short distance range of below 3 nm by using a Cy2-Cy7 pair having extremely small spectral overlap. Using two DNA duplexes with a small difference in the labeling position, we demonstrated that our assay can observe subtle changes at a short distance range. High sensitivity in the range of 1-3 nm and compatibility with the conventional FRET assay make this approach useful for understanding dynamics at a short distance.

Indexed as

DNAFluorescence Resonance Energy TransferNanotechnologyFluorescenceDNAconformational dynamicsfluorescenceFRET probesingle-molecule FRET

Identifiers

PMID33171642
PMCPMC7695202
OpenAlexW3102975347

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.