ArticleInfectious diseases and therapy2020
Etiology of Severe Community-Acquired Pneumonia in Adults Based on Metagenomic Next-Generation Sequencing: A Prospective Multicenter Study.
Article in Infectious diseases and therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers, 4 of them syntheses that pooled it.
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116 citing papers in PubMed, 4 syntheses or guidelines pooled it, 149 citations in OpenAlex.
- Gestational psittacosis: a systematic review of clinical manifestations and outcomes.BMC infectious diseases · 2026Pooled it
- Global prevalence of psittacosis in outbreaks: a systematic review and meta-analysis.BMC public health · 2025Pooled it
- Prevalence of atypical pathogens in patients with severe pneumonia: a systematic review and meta-analysis.BMJ open · 2023Pooled it
- Clinical values of metagenomic next-generation sequencing in patients with severe pneumonia: a systematic review and meta-analysis.Frontiers in cellular and infection microbiology · 2023Pooled it
- Rapid diagnosis ofExperimental and therapeutic medicine · 2026Article
- A drug-repurposing nanotheranostic for synergistic management of resistant bacterial pneumonia.Asian journal of pharmaceutical sciences · 2026Article
- Benchmarking next- versus third-generation sequencing in metagenomics: performance metrics and diagnostic efficacy.Microbiology spectrum · 2026Article
- Computed tomography findings and severity scores in Chlamydia psittaci pneumonia: a retrospective study of 69 cases with clinical correlation.BMC infectious diseases · 2026Article
- Targeted next-generation sequencing improves diagnosis and antimicrobial stewardship in Chlamydia psittaci pneumonia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- The impact of the timing of mNGS-guided antibiotic adjustment on clinical outcomes in ICU patients with severe community-acquired pneumonia: a retrospective study.Annals of clinical microbiology and antimicrobials · 2026Article
- Syndromic molecular testing in mechanically ventilated patients with severe pneumonia: a supportive diagnostic approach.Microbiology spectrum · 2026Article
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- Clinical Characteristics and Machine Learning-Based Severity Classification ofInfection and drug resistance · 2026Article
- Clinical features and prognostic factors ofFrontiers in medicine · 2026Article
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- Clinical Features of 186 Cases ofInfection and drug resistance · 2026Article
- Clinical Features and Risk Factors for Severe Disease in 57 Cases ofInfection and drug resistance · 2026Article
- Metagenomic next-generation sequencing improves diagnosis ofFrontiers in medicine · 2026Article
56 more citing papers are in PubMed but not listed here.
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11 authors at 8 institutions in 1 country.
Funding
Abstract
backgroundMetagenomic next-generation sequencing (mNGS) of bronchoalveolar lavage fluid (BALF) has the potential to improve the pathogen identification in severe community-acquired pneumonia (SCAP).
methodsIn this 1.5-year, multicenter, prospective study, we investigated the usefulness of mNGS of BALF for identifying pathogens of SCAP in hospitalized adults, comparing it with other laboratory methods.
resultsOf 329 SCAP adults, a microbial etiology was established in 304 cases (92.4%). The overall microbial yield was 90.3% for mNGS versus 39.5% for other methods (P < 0.05). The most frequently detected pathogens in immunocompetent patients were Streptococcus pneumoniae (14.8%), rhinovirus (9.8%), Haemophilus influenzae (9.1%), Staphylococcus aureus (8.7%), and Chlamydia psittaci (8.0%), while in immunocompromised patients they were Pneumocystis jirovecii (44.6%), Klebsiella pneumoniae (18.5%), Streptococcus pneumoniae (15.4%), Haemophilus influenzae (13.8%), and Pseudomonas aeruginosa (13.8%). Notably, novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was identified from two patients solely by mNGS in January 2020; uncommon pathogens including Orientia tsutsugamushi and Nocardia otitidiscaviarum were identified from one patient, respectively. Furthermore, mixed infections were detected in 56.8% of the patients.
conclusionsA high microbial detection rate was achieved in SCAP adults using mNGS testing of BALF. The most frequently detected pathogens of SCAP differed between immunocompetent and immunocompromised patients. mNGS testing may be an powerful tool for early identification of potential pathogens for SCAP to initiate a precise antimicrobial therapy.
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