ArticleIn vitro cellular & developmental biology. Animal2020
The microfollicle: a model of the human hair follicle for in vitro studies.
Article in In vitro cellular & developmental biology. Animal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Beyond conventional PRP: a rationale for bioengineered, growth-factor-defined platelet mimetics in alopecia-the precision re-engineered efficacy optimization framework.Stem cell research & therapy · 2026Article
- Synergistic effects of mesenchymal stem cell secretome and Wnt10b on hair follicle regeneration in a 3D bioengineering model: a preclinical study.Stem cell research & therapy · 2025Article
- Synergizing biomaterials and cellular molecular pathways: a new frontier in hair follicle generation.Journal of translational medicine · 2025Review
- Regenerative Hair Pigmentation via Skin Organoids: Adaptive Patterning Mediated by Collagen VI and Semaphorin 3C.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Ideal Living Skin Equivalents, From Old Technologies and Models to Advanced Ones: The Prospects for an Integrated Approach.BioMed research international · 2024Review
- Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics.Scientific reports · 2023Article
- The dermal papilla dilemma and potential breakthroughs in bioengineering hair follicles.Cell and tissue research · 2023Review
- Bioengineering of Hair Follicle-like Structure for Validation of Hair Growth Promoting Compounds.Bioengineering (Basel, Switzerland) · 2022Article
- The Emergent Power of Human Cellular vs Mouse Models in Translational Hair Research.Stem cells translational medicine · 2022Article
- Self-assembled complete hair follicle organoids by coculture of neonatal mouse epidermal cells and dermal cells in Matrigel.Annals of translational medicine · 2022Article
Corrections and comments
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Authors and funding
16 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Access to complex in vitro models that recapitulate the unique markers and cell-cell interactions of the hair follicle is rather limited. Creation of scalable, affordable, and relevant in vitro systems which can provide predictive screens of cosmetic ingredients and therapeutic actives for hair health would be highly valued. In this study, we explore the features of the microfollicle, a human hair follicle organoid model based on the spatio-temporally defined co-culture of primary cells. The microfollicle provides a 3D differentiation platform for outer root sheath keratinocytes, dermal papilla fibroblasts, and melanocytes, via epidermal-mesenchymal-neuroectodermal cross-talk. For assay applications, microfollicle cultures were adapted to 96-well plates suitable for medium-throughput testing up to 21 days, and characterized for their spatial and lineage markers. The microfollicles showed hair-specific keratin expression in both early and late stages of cultivation. The gene expression profile of microfollicles was also compared with human clinical biopsy samples in response to the benchmark hair-growth compound, minoxidil. The gene expression changes in microfollicles showed up to 75% overlap with the corresponding gene expression signature observed in the clinical study. Based on our results, the cultivation of the microfollicle appears to be a practical tool for generating testable insights for hair follicle development and offers a complex model for pre-clinical substance testing.
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