Evidence map›Paper›PMID 33170392›Full record

ArticleJournal of assisted reproduction and genetics2021

LINE-1 retrotransposon methylation in chorionic villi of first trimester miscarriages with aneuploidy.

Stanislav A Vasilyev, Ekaterina N Tolmacheva, Oksana Yu Vasilyeva, Anton V Markov, Daria I Zhigalina, Lada A Zatula, Vasilissa A Lee, Ekaterina S Serdyukova, Elena A Sazhenova, Tatyana V Nikitina and 2 more

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In one paragraph

Article in Journal of assisted reproduction and genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
1.6field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
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  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Stanislav A VasilyevResearch Institute of Medical Genetics, Tomsk National Research Medical Center, Nab. R. Ushaiki, 10, Tomsk, Russia. stanislav.vasilyev@medgenetics.ru.ORCID http://orcid.org/0000-0002-5301-070X
Ekaterina N TolmachevaResearch Institute of Medical Genetics, Tomsk National Research Medical Center, Nab. R. Ushaiki, 10, Tomsk, Russia.ORCID https://orcid.org/0000-0001-6427-3276
Oksana Yu VasilyevaResearch Institute of Medical Genetics, Tomsk National Research Medical Center, Nab. R. Ushaiki, 10, Tomsk, Russia.ORCID https://orcid.org/0000-0001-5797-0014
Anton V MarkovResearch Institute of Medical Genetics, Tomsk National Research Medical Center, Nab. R. Ushaiki, 10, Tomsk, Russia.ORCID https://orcid.org/0000-0002-5824-6439
Daria I ZhigalinaResearch Institute of Medical Genetics, Tomsk National Research Medical Center, Nab. R. Ushaiki, 10, Tomsk, Russia.ORCID https://orcid.org/0000-0003-2800-8875
Lada A ZatulaDepartment of Medical Genetics, Siberian State Medical University, Moskovskiy Trakt, 2, Tomsk, Russia.
Vasilissa A LeeDepartment of Medical Genetics, Siberian State Medical University, Moskovskiy Trakt, 2, Tomsk, Russia.
Ekaterina S SerdyukovaBiological Institute, National Research Tomsk State University, Lenina, 36, Tomsk, Russia.
Elena A SazhenovaResearch Institute of Medical Genetics, Tomsk National Research Medical Center, Nab. R. Ushaiki, 10, Tomsk, Russia.ORCID https://orcid.org/0000-0003-3875-3932
Tatyana V NikitinaResearch Institute of Medical Genetics, Tomsk National Research Medical Center, Nab. R. Ushaiki, 10, Tomsk, Russia.ORCID https://orcid.org/0000-0002-4230-6855
Anna A KashevarovaResearch Institute of Medical Genetics, Tomsk National Research Medical Center, Nab. R. Ushaiki, 10, Tomsk, Russia.ORCID https://orcid.org/0000-0002-0716-4302
Igor N LebedevResearch Institute of Medical Genetics, Tomsk National Research Medical Center, Nab. R. Ushaiki, 10, Tomsk, Russia.ORCID https://orcid.org/0000-0002-0482-8046
Tomsk National Research Medical Center · RUSiberian State Medical University · RUNational Research Tomsk State University · RU

Funding

Russian Science Foundation 19-74-10026
6 · The paper itself

Abstract

purposeHigh frequency of aneuploidy in meiosis and cleavage stage coincides with waves of epigenetic genome reprogramming that may indicate a possible association between epigenetic mechanisms and aneuploidy occurrence. This study aimed to assess the methylation level of the long interspersed repeat element 1 (LINE-1) retrotransposon in chorionic villi of first trimester miscarriages with a normal karyotype and aneuploidy.

methodsThe methylation level was assessed at 19 LINE-1 promoter CpG sites in chorionic villi of 141 miscarriages with trisomy of chromosomes 2, 6, 8-10, 13-15, 16, 18, 20-22, and monosomy X using massive parallel sequencing.

resultsThe LINE-1 methylation level was elevated statistically significant in chorionic villi of miscarriages with both trisomy (45.2 ± 4.3%) and monosomy X (46.9 ± 4.2%) compared with that in induced abortions (40.0 ± 2.4%) (p < 0.00001). The LINE-1 methylation levels were specific for miscarriages with different aneuploidies and significantly increased in miscarriages with trisomies 8, 14, and 18 and monosomy X (p < 0.05). The LINE-1 methylation level increased with gestational age both for group of miscarriages regardless of karyotype (R = 0.21, p = 0.012) and specifically for miscarriages with trisomy 16 (R = 0.48, p = 0.007). LINE-1 methylation decreased with maternal age in miscarriages with a normal karyotype (R = - 0.31, p = 0.029) and with trisomy 21 (R = - 0.64, p = 0.024) and increased with paternal age for miscarriages with trisomy 16 (R = 0.38, p = 0.048) and monosomy X (R = 0.73, p = 0.003).

conclusionOur results indicate that the pathogenic effects of aneuploidy in human embryogenesis can be supplemented with significant epigenetic changes in the repetitive sequences.

Indexed as

Abortion, SpontaneousAdultAneuploidyChorionic VilliDNA MethylationEmbryonic DevelopmentFemaleHumansLong Interspersed Nucleotide ElementsPregnancyPregnancy Trimester, FirstAneuploidyDNA methylationLINE-1 retrotransposonMiscarriageParental age

Identifiers

PMID33170392
PMCPMC7823001
OpenAlexW3100072693

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.