ArticleJournal of proteome research2020
Enhanced Validation of Antibodies Enables the Discovery of Missing Proteins.
Article in Journal of proteome research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- A high-resolution spatial map of cilia-associated proteins in the human fallopian tube.Nature communications · 2026Article
- A High-Resolution Subcellular Map of Proteins in Cells with Motile Cilia.Journal of proteome research · 2026Article
- Clinicopathologic Characteristics of Trop Family Proteins (Trop-2 and EpCAM) in Gastric Carcinoma.Journal of gastric cancer · 2024Article
- Antibody characterization is critical to enhance reproducibility in biomedical research.eLife · 2024Review
- Matrix metalloproteinases in intestinal fibrosis.Journal of Crohn's & colitis · 2024Review
- Multiplexed selectivity screening of anti-GPCR antibodies.Science advances · 2023Article
- Spatial Proteomics for Further Exploration of Missing Proteins: A Case Study of the Ovary.Journal of proteome research · 2023Article
- The human protein atlas-Integrated omics for single cell mapping of the human proteome.Protein science : a publication of the Protein Society · 2023Article
- Meningioma classification by immunohistochemistry: A replicability study.Brain & spine · 2023Article
- Protein Expression Profile of ACE2 in the Normal and COVID-19-Affected Human Brain.Journal of proteome research · 2022Article
- Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level.Nature communications · 2022Article
- Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads.Metabolites · 2022Article
- Genome-wide annotation of protein-coding genes in pig.BMC biology · 2022Article
- Progress Identifying and Analyzing the Human Proteome: 2021 Metrics from the HUPO Human Proteome Project.Journal of proteome research · 2021Article
- Validation of antibodies: Lessons learned from the Common Fund Protein Capture Reagents Program.Science advances · 2021Article
- Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLCell reports · 2021Article
- Best Practices for Technical Reproducibility Assessment of Multiplex Immunofluorescence.Frontiers in molecular biosciences · 2021Review
- FAM21C Promotes Hepatocellular Carcinoma Invasion and Metastasis by Driving Actin Cytoskeleton RemodelingFrontiers in oncology · 2021Article
- Research on the Human Proteome Reaches a Major Milestone: >90% of Predicted Human Proteins Now Credibly Detected, According to the HUPO Human Proteome Project.Journal of proteome research · 2020Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The localization of proteins at a tissue- or cell-type-specific level is tightly linked to the protein function. To better understand each protein's role in cellular systems, spatial information constitutes an important complement to quantitative data. The standard methods for determining the spatial distribution of proteins in single cells of complex tissue samples make use of antibodies. For a stringent analysis of the human proteome, we used orthogonal methods and independent antibodies to validate 5981 antibodies that show the expression of 3775 human proteins across all major human tissues. This enhanced validation uncovered 56 proteins corresponding to the group of "missing proteins" and 171 proteins of unknown function. The presented strategy will facilitate further discussions around criteria for evidence of protein existence based on immunohistochemistry and serves as a useful guide to identify candidate proteins for integrative studies with quantitative proteomics methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.