ArticleJournal of veterinary internal medicine2020
Effect of dilution of canine blood samples on the specificity of saline agglutination tests for immune-mediated hemolysis.
Article in Journal of veterinary internal medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 17 citations in OpenAlex.
- Immune-mediated hemolytic anemia in a bottlenose dolphin: case report and literature review.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Review
- Preliminary Study on Treatment Outcomes and Prednisolone Tapering after Marine Lipid Extract EAB-277 Supplementation in Dogs with Immune-Mediated Hemolytic Anemia.Veterinary sciences · 2023Article
- Article
- The Indirect Antiglobulin (Coombs') Test Is Specific but Less Sensitive Than the Direct Antiglobulin Test for Detecting Anti-Erythrocytic Autoantibodies and Thereby Immune-Mediated Hemolytic Anemia in Dogs.Veterinary sciences · 2023Article
- Validation of a cage-side agglutination card for Dal blood typing in dogs.Journal of veterinary internal medicine · 2023Article
- Retrospective analysis of immunosuppressive and anti-thrombotic protocols in nonassociative immune mediated hemolytic anemia in dogs.Journal of veterinary internal medicine · 2023Article
- Comparative study of immunohematological tests with canine blood samples submitted for a direct antiglobulin (Coombs') test.Canine medicine and genetics · 2021Article
- Letter regarding "Effect of dilution of canine blood samples on the specificity of saline agglutination tests for immune-mediated hemolysis," original and modified saline agglutination tests vs direct Coombs' tests.Journal of veterinary internal medicine · 2021Article
- Response to letter regarding "Effect of dilution of canine blood samples on the specificity of saline agglutination tests for immune-mediated hemolysis".Journal of veterinary internal medicine · 2021Article
- Effect of dilution of canine blood samples on the specificity of saline agglutination tests for immune-mediated hemolysis.Journal of veterinary internal medicine · 2020Article
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2 authors at 2 institutions in 1 country.
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Abstract
backgroundSaline agglutination tests (SATs) are widely recommended for diagnosis of immune-mediated hemolytic anemia in dogs, but there are frequent false-positive results.
objectivesSpecificity of SATs will improve at higher saline-to-blood ratios. ANIMALS: One hundred fifty dogs treated at a veterinary referral hospital with hematocrits ≤30%.
methodsProspective diagnostic accuracy study. Immune-mediated hemolysis (IMH) was considered present if a gel direct antiglobulin test (DAT) was positive and there was clinical evidence of hemolysis (n = 9), absent if another mechanism for anemia was identified and the DAT was negative or there was no hemolysis (n = 138), and if IMH status was unclear, dogs were excluded (n = 3). Saline agglutination tests were prepared at 1 : 1, 4 : 1, 9 : 1, and 49 : 1 saline-to-blood ratios, and microscopic agglutination was considered a positive result.
resultsSpecificity for IMH increased from 29% (95% confidence interval 20-38) at a 1 : 1 dilution to 97% (93-99) at a 49 : 1 dilution. Sensitivity was 88% (47-100) at 1 : 1 and 4 : 1 dilutions and 67% (30-93%) at 9 : 1 and 49 : 1 dilutions. Diagnostic accuracy increased from 33% (24-42) at 1 : 1 dilution to 95% (90-98) at 49 : 1 dilution. CONCLUSIONS AND CLINICAL IMPORTANCE: If performed using a 49 : 1 saline-to-blood ratio, SATs achieve high specificity for IMH. Based on a gold standard of positive DAT and evidence of hemolysis, lower saline-to-blood ratio results should not be used because false-positive results are common.
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