ArticleElectrophoresis2021
A novel method of high-purity extracellular vesicle enrichment from microliter-scale human serum for proteomic analysis.
Article in Electrophoresis, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Osmotic Remodeling of Extracellular Vesicles for Precision Nanomedicine.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Repeatability and reproducibility of chromatographic parameters and resulting figures of merit on polyester C-CP fiber columns for the routine isolation and analysis of HEK293-derived extracellular vesicles.Analytical methods : advancing methods and applications · 2026Article
- Comparison of methods for isolation of extracellular vesicles from bronchoalveolar lavage fluid.Extracellular vesicles and circulating nucleic acids · 2026Article
- Extracellular vesicles, including large translating vesicles called midbody remnants, are released during the cell cycle.Molecular biology of the cell · 2024Article
- Isolation and quantification of human urinary exosomes using a Tween-20 elution solvent from polyester, capillary-channeled polymer fiber columns.Analytica chimica acta · 2024Article
- In-line coupling of capillary-channeled polymer fiber columns with optical absorbance and multi-angle light scattering detection for the isolation and characterization of exosomes.Analytical and bioanalytical chemistry · 2024Article
- MicroRNA Nano-Shuttles: Engineering Extracellular Vesicles as a Cutting-Edge Biotechnology Platform for Clinical Use in Therapeutics.Biological procedures online · 2024Review
- In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.Journal of proteome research · 2024Article
- David M. Lubman-The University of Michigan-A retrospective in research.Mass spectrometry reviews · 2023Review
- Rapid isolation and quantification of extracellular vesicles from suspension-adapted human embryonic kidney cells using capillary-channeled polymer fiber spin-down tips.Electrophoresis · 2023Article
- Comparison of the capillary-channeled polymer (C-CP) fiber spin-down tip approach to traditional methods for the isolation of extracellular vesicles from human urine.Analytical and bioanalytical chemistry · 2022Article
- Review
- Column-based Technology for CD9-HPLC Immunoaffinity Isolation of Serum Extracellular Vesicles.Journal of proteome research · 2021Article
- Rapid separation of blood plasma exosomes from low-density lipoproteins via a hydrophobic interaction chromatography method on a polyester capillary-channeled polymer fiber phase.Analytica chimica acta · 2021Article
- The role of lipids in exosome biology and intercellular communication: Function, analytics and applications.Traffic (Copenhagen, Denmark) · 2021Review
- Blood Nanoparticles - Influence on Extracellular Vesicle Isolation and Characterization.Frontiers in pharmacology · 2021Review
- Exosome-Based Liquid Biopsy in Early Screening and Diagnosis of Cancers.Dose-response : a publication of International Hormesis SocietyReview
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
We have developed a rapid, low-cost, and simple separation strategy to separate extracellular vesicles (EVs) from a small amount of serum (i.e.,<100 μL) with minimal contamination by serum proteins and lipoprotein particles to meet the high purity requirement for EV proteome analysis. EVs were separated by a novel polyester capillary channel polymer (PET C-CP) fiber phase/hydrophobic interaction chromatography (HIC) method which is rapid and can process small size samples. The collected EV fractions were subjected to a post-column cleanup protocol using a centrifugal filter to perform buffer exchange and eliminate potential coeluting non-EV proteins while minimizing EV sample loss. Downstream characterization demonstrated that our current strategy can separate EVs with the anticipated exosome-like particle size distribution and high yield (∼1 × 10
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.