Evidence map›Paper›PMID 33169228›Full record

ArticleAMB Express2020

HPLC phenolic profile and induction of apoptosis by Linum usitatissimum extract in LNCaP cells by caspase3 and Bax pathways.

Xin Zhou, Ningou Huang, Wenxin Chen, Tang Xiaoling, Behnam Mahdavi, Amir Raoofi, Davood Mahdian, Hadi Atabati

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.7field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 2 countries.

Xin ZhouDepartment of Urology, Beijing Tiantan Hospital,Capital Medical University, Beijing, 100070, China.
Ningou HuangDepartment of Pharmacy, The Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, 210031, Jiangsu, China.
Wenxin ChenDepartment of Urology, Occupational Disease Hospital of Xinjiang Uygur Autonomous Region, Urumqi, 830000, Xinjiang, China.
Tang XiaolingJiangxi Research Institute of Traditional Chinese Medicine, Nanchang, 330046, Jiangxi, China. txl123666@sina.com.ORCID http://orcid.org/0000-0002-0977-6396
Behnam MahdaviDepartment of Chemistry, Faculty of Science, Hakim Sabzevari University, 96179-76487, Sabzevar, Iran.
Amir RaoofiCellular and Molecular Research Center, Department of Anatomy, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Davood MahdianCellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Hadi AtabatiCellular and Molecular Research Center, Department of Anatomy, Sabzevar University of Medical Sciences, Sabzevar, Iran.
Sabzevar University of Medical Sciences · IRCapital Medical University · CNHakim Sabzevari University · IRJiangxi University of Traditional Chinese Medicine · CNPeople's Hospital of Xinjiang Uygur Autonomous Region · CNSecond Affiliated Hospital of Nanjing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Linum usitatissimum is a candidate as a remedy to treat prostate problems in some folklore medicines. In this study, we have reported the phenolic and flavonoid constituents, antioxidant activity, and potential of the plant extract against prostate cancer cells. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. While the total phenolic and flavonoid content (TPC and TFC) were analyzed using classic methods. The antioxidant activity of the extract was also evaluated. MTT assay and flow cytometry technique was used to evaluate antiproliferation activity and induction apoptosis of the plant extract on prostate cancer cells of LNCaP. We also evaluated the gene expression of Bax and caspase-3 using the real-time qPCR assay. HPLC result revealed that L. usitatissimum extract (LUE) was rich in phenolic acids such as gallic, ferulic, and vanillic acid with the amount of 3.56, 2.12, 1.24 μg/g extract respectively. 383.4 mg GAE/g and 47.1 mgRuE/g were calculated for total phenolic and flavonoid content. LUE exhibited radical scavenging activity with IC

Indexed as

BaxCaspase-3Induction apoptosisLinum usitatissimumPhenolic profileProstate cancer

Identifiers

PMID33169228
PMCPMC7652951
OpenAlexW3098044989

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.