ArticleScientific data2020
Paired rRNA-depleted and polyA-selected RNA sequencing data and supporting multi-omics data from human T cells.
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Article in Scientific data, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20citing papers in PubMed
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Who cites it
20 citing papers in PubMed.
- Beyond blacklists: a critical assessment of exclusion set generation strategies and alternative approaches.Bioinformatics (Oxford, England) · 2026Article
- Detection of alternative splicing: deep sequencing or deep learning?Briefings in bioinformatics · 2026Article
- Comparison of automated and manual mRNA enrichment to automated rRNA depletion for whole-blood RNA-sequencing.Scientific reports · 2025Article
- Transcriptomic detection of Candidatus Allocryptoplasma (Anaplasmataceae) in Galápagos marine iguanas (Amblyrhynchus cristatus, Iguanidae).Parasites & vectors · 2025Article
- Beyond Blacklists: A Critical Assessment of Exclusion Set Generation Strategies and Alternative Approaches.bioRxiv : the preprint server for biology · 2025Article
- Construction of RNA reference materials for improving the quantification of transcriptomic data.Nature protocols · 2025Review
- Preservation of milk in liquid nitrogen during sample collection does not affect the RNA quality for RNA-seq analysis.BMC genomics · 2025Article
- Benchmark of cellular deconvolution methods using a multi-assay dataset from postmortem human prefrontal cortex.Genome biology · 2025Article
- Detection of viral contamination in cell lines using ViralCellDetector.Frontiers in microbiology · 2025Article
- LINE1 modulate human T cell function by regulating protein synthesis during the life span.Science advances · 2024Article
- Article
- CRISPR activation to characterize splice-altering variants in easily accessible cells.American journal of human genetics · 2024Article
- An integrative pipeline for circular RNA quantitative trait locus discovery with application in human T cells.Bioinformatics (Oxford, England) · 2023Article
- Transcriptomic and lipidomic profiling of subcutaneous and visceral adipose tissues in 15 vertebrates.Scientific data · 2023Article
- A cap 0-dependent mRNA capture method to analyze the yeast transcriptome.Nucleic acids research · 2022Article
- How Machine Learning and Statistical Models Advance Molecular Diagnostics of Rare Disorders Via Analysis of RNA Sequencing Data.Frontiers in molecular biosciences · 2021Review
- Deregulation of ncRNA in Neurodegenerative Disease: Focus on circRNA, lncRNA and miRNA in Amyotrophic Lateral Sclerosis.Frontiers in genetics · 2021Review
- Comparative Analysis of the Circular Transcriptome in Muscle, Liver, and Testis in Three Livestock Species.Frontiers in genetics · 2021Article
- Paired rRNA-depleted and polyA-selected RNA sequencing data and supporting multi-omics data from human T cells.Scientific data · 2020Article
- Biological mass spectrometry enables spatiotemporal 'omics: From tissues to cells to organelles.Mass spectrometry reviewsReview
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Authors and funding
15 authors.
Li ChenKey Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, Department of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Second Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Ruirui YangKey Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, Department of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Second Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Tony KwanHuman Genetics, McGill University, 740 Dr. Penfield, Montreal, QC, H3A 0G1, Canada.ORCID 0000-0001-8929-2334
Chao TangKey Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, Department of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Second Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Stephen WattDepartment of Human Genetics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH, UK.
Yiming ZhangKey Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, Department of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Second Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Guillaume BourqueHuman Genetics, McGill University, 740 Dr. Penfield, Montreal, QC, H3A 0G1, Canada.ORCID 0000-0002-3933-9656
Bing GeHuman Genetics, McGill University, 740 Dr. Penfield, Montreal, QC, H3A 0G1, Canada.
Kate DownesDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Long Road, Cambridge, CB2 0PT, UK.
Mattia FrontiniDepartment of Haematology, University of Cambridge, Cambridge Biomedical Campus, Long Road, Cambridge, CB2 0PT, UK.
Willem H OuwehandDepartment of Human Genetics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH, UK.ORCID 0000-0002-7744-1790
Jing-Wen LinKey Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, Department of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Second Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.ORCID 0000-0003-3338-8788
Nicole SoranzoDepartment of Human Genetics, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1HH, UK. ns6@sanger.ac.uk.ORCID 0000-0003-1095-3852
Tomi PastinenCenter for Pediatric Genomic Medicine, Children's Mercy Kansas City, 2401 Gilham Rd., Kansas City, 64108 MO, USA. tpastinen@cmh.edu.
Lu ChenKey Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, Department of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Second Hospital, Sichuan University, Chengdu, Sichuan, 610041, China. luchen@scu.edu.cn.
Funding
British Heart Foundation FS/18/53/33863EC | EC Seventh Framework Programm | FP7 Health (FP7-HEALTH - Specific Programme "Cooperation": Health) HEALTH-F5-2011-282510Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) EP1-120608,EP2-120609
Abstract
Both poly(A) enrichment and ribosomal RNA depletion are commonly used for RNA sequencing. Either has its advantages and disadvantages that may lead to biases in the downstream analyses. To better access these effects, we carried out both ribosomal RNA-depleted and poly(A)-selected RNA-seq for CD4
Indexed as
Sequence Analysis, RNAAlternative SplicingEpigenomicsGene ExpressionGene LibraryGenomicsHumansPoly AQuantitative Trait LociRNA, RibosomalT-LymphocytesPoly ARNA, Ribosomal
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.