ArticleMicrobial cell factories2020
Construction and description of a constitutive plipastatin mono-producing Bacillus subtilis.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 64 citations in OpenAlex.
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- Antioxidant Properties of Biosurfactants: Multifunctional Biomolecules with Added Value in Formulation Chemistry.Biomolecules · 2025Review
- Genetic Code Expansion for Controlled Surfactin Production in a High Cell-DensityMicroorganisms · 2025Article
- Recombinant Production of Bovine αMicroorganisms · 2025Article
- In Vivo Quantification of Surfactin Nonribosomal Peptide Synthetase Complexes inMicroorganisms · 2024Article
- The influence of growth rate-controlling feeding strategy on the surfactin production in Bacillus subtilis bioreactor processes.Microbial cell factories · 2024Article
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- The Utilization ofMicroorganisms · 2024Article
- An Integrated Pipeline and Overexpression of a Novel Efflux Transporter, YoeA, Significantly Increases Plipastatin Production inFoods (Basel, Switzerland) · 2024Article
- Strategies for improving fengycin production: a review.Microbial cell factories · 2024Review
- Metabolic insights from mass spectrometry imaging of biofilms: A perspective from model microorganisms.Methods (San Diego, Calif.) · 2024Review
- An insight into the utilization of microbial biosurfactants pertaining to their industrial applications in the food sector.Food science and biotechnology · 2024Review
- Antimicrobial peptides from Bacillus spp. and strategies to enhance their yield.Applied microbiology and biotechnology · 2023Review
- Deletion of Rap-phosphatases for quorum sensing control in Bacillus and its effect on surfactin production.AMB Express · 2023Article
- Exploring the Potential Molecular Mechanisms of Interactions between a Probiotic Consortium and Its Coral Host.mSystems · 2023Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPlipastatin is a potent Bacillus antimicrobial lipopeptide with the prospect to replace conventional antifungal chemicals for controlling plant pathogens. However, the application of this lipopeptide has so far been investigated in a few cases, principally because of the yield in low concentration and unknown regulation of biosynthesis pathways. B. subtilis synthesizes plipastatin by a non-ribosomal peptide synthetase encoded by the ppsABCDE operon. In this study, B. subtilis 3NA (a non-sporulation strain) was engineered to gain more insights about plipastatin mono-production.
resultsThe 4-phosphopantetheinyl transferase Sfp posttranslationally converts non-ribosomal peptide synthetases from inactive apoforms into their active holoforms. In case of 3NA strain, sfp gene is inactive. Accordingly, the first step was an integration of a repaired sfp version in 3NA to construct strain BMV9. Subsequently, plipastatin production was doubled after integration of a fully expressed degQ version from B. subtilis DSM10
conclusionsThis study provides evidence that degQ stimulates the native plipastatin production. Moreover, a full plipastatin production requires surfactin synthetase or some of its components. Furthermore, as another conclusion of this study, results point towards ornithine provision being an indispensable constituent for a plipastatin mono-producer B. subtilis strain. Therefore, targeting the ornithine metabolic flux might be a promising strategy to further investigate and enhance plipastatin production by B. subtilis plipastatin mono-producer strains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.