Evidence map›Paper›PMID 33165701›Full record

ArticleActa parasitologica2021

Immune Response of Mice Against Babesia canis Antigens is Enhanced When Antigen is Coupled to Gold Nanoparticles.

S A Staroverov, A S Fomin, S V Kozlov, A A Volkov, E S Kozlov, K P Gabalov, L A Dykman

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Article in Acta parasitologica, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

S A StaroverovInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, Russia, 410049.
A S FominInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, Russia, 410049.
S V KozlovSaratov State Vavilov Agrarian University, 1 Teatralnaya Ploshchad, Saratov, Russia, 410012.
A A VolkovSaratov State Vavilov Agrarian University, 1 Teatralnaya Ploshchad, Saratov, Russia, 410012.
E S KozlovSaratov State Vavilov Agrarian University, 1 Teatralnaya Ploshchad, Saratov, Russia, 410012.
K P GabalovInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, Russia, 410049.
L A DykmanInstitute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, Russia, 410049. dykman_l@ibppm.ru.ORCID http://orcid.org/0000-0003-2440-6761
Institute of Biochemistry and Physiology of Plants and Microorganisms · RUSaratov State Agrarian University named after N.I .Vavilov · RU

Funding

Russian Science Foundation 19-14-00077
6 · The paper itself

Abstract

purposeThe aim of this study was to isolate Babesia canis soluble antigens and to investigate the effect of their conjugates with gold nanoparticles on the immunogenicity in laboratory animals.

methodsA procedure was developed for isolating and purifying B. canis antigens. The isolated culture antigen of B. canis 495 was coupled to gold nanoparticles, and the conjugate was used to immunize laboratory mice.

resultsWestern blotting showed that the resultant antiserum specifically recognized the proteins of the B. canis strains isolated from naturally infected dogs. The antibody titer, the respiratory activity of peritoneal macrophages, the proliferative activity of splenocytes, and the production of cytokines were maximal when the animals were immunized with the antigen-nanoparticle conjugate emulsified in complete Freund's adjuvant. Without adjuvant, the babesial antigen was weakly immunogenic.

conclusionTherefore, the use of gold nanoparticles as an antigen carrier induced a broad immune response involving both cellular and humoral responses. The antibodies raised by the proposed procedure are potentially effective at immunodetection of Babesia canis infections in dogs.

Indexed as

BabesiaBabesiosisDog DiseasesMetal NanoparticlesAnimalsDogsGoldImmunityMiceGoldAntigenBabesia canisGold nanoparticlesImmunizationProtective immunityVaccine

Identifiers

PMID33165701
OpenAlexW3101310421

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.