ReviewFrontiers in bioengineering and biotechnology2020
Nonlinear Optical Microscopy: From Fundamentals to Applications in Live Bioimaging.
Review in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- All-optical control of second-harmonic generation in β-BaBNature communications · 2026Article
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- Photoisomerizing molecules in biological membranes.Nature reviews. Chemistry · 2026Review
- Stimulated Raman Scattering Imaging Enabled Biomarker Discovery for Precision Medicine.Chemical & biomedical imaging · 2025Review
- Fast multimodal imaging combined with machine learning identifying taurine as a potential marker for breast cancer margin assessment.NPJ digital medicine · 2025Article
- Label-free histological identification of intraductal carcinoma of the prostate using texture analysis-based multimodal stimulated Raman scattering microscopy.Scientific reports · 2025Article
- Light and metabolism: label-free optical imaging of metabolic activities in biological systems [Invited].Biomedical optics express · 2025Review
- Pair Natural Orbitals for Coupled Cluster Quadratic Response Theory.The journal of physical chemistry. A · 2025Article
- Video microscopy: an old story with a bright biological future.Biomedical engineering online · 2025Review
- Current Trends and Advances in Nanoplatforms-Based Imaging for Cancer Diagnosis.Indian journal of microbiology · 2025Article
- Raman Spectroscopy in Cellular and Tissue Aging Research.Aging cell · 2025Review
- Dynamic imaging of myelin pathology in physiologically preserved human brain tissue using third harmonic generation microscopy.PloS one · 2025Article
- Review
- Direct Observation of Circularly Polarized Nonlinear Optical Activities in Chiral Hybrid Lead Halides.Journal of the American Chemical Society · 2024Article
- Quantitative phase imaging by gradient retardance optical microscopy.Scientific reports · 2024Article
- Double-Clad Antiresonant Hollow-Core Fiber and Its Comparison with Other Fibers for Multiphoton Micro-Endoscopy.Sensors (Basel, Switzerland) · 2024Article
- Numerical study of transient absorption saturation in single-layer graphene for optical nanoscopy applications.Scientific reports · 2024Article
- Imagining the future of optical microscopy: everything, everywhere, all at once.Communications biology · 2023Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A recent challenge in the field of bioimaging is to image vital, thick, and complex tissues in real time and in non-invasive mode. Among the different tools available for diagnostics, nonlinear optical (NLO) multi-photon microscopy allows label-free non-destructive investigation of physio-pathological processes in live samples at sub-cellular spatial resolution, enabling to study the mechanisms underlying several cellular functions. In this review, we discuss the fundamentals of NLO microscopy and the techniques suitable for biological applications, such as two-photon excited fluorescence (TPEF), second and third harmonic generation (SHG-THG), and coherent Raman scattering (CRS). In addition, we present a few of the most recent examples of NLO imaging employed as a label-free diagnostic instrument to functionally monitor
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