ArticleApplications in plant sciences2020
A simple high-throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves.
Article in Applications in plant sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Rice straw-derived smoke water promotes rice root growth under phosphorus deficiency by modulating oxidative stress and photosynthetic gene expression.Scientific reports · 2023Article
- Hyperspectral and genome-wide association analyses of leaf phosphorus status in local Thai indica rice.PloS one · 2022Article
- A Colorimetric Dip Strip Assay for Detection of Low Concentrations of Phosphate in Seawater.Sensors (Basel, Switzerland) · 2021Article
- A simple high-throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves.Applications in plant sciences · 2020Article
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3 authors.
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Abstract
premisePhosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high-throughput colorimetric microplate technique to measure Pi contents in rice ( METHODS AND
resultsWe used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time-consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply.
conclusionsCompared with existing techniques, this protocol is more suited to an initial screening, enabling one researcher to determine the Pi contents of thousands of rice leaf samples within a few hours.
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