Capsaicin acts through tNOX (ENOX2) to induce autophagic apoptosis in p53-mutated HSC-3 cells but autophagy in p53-functional SAS oral cancer cells.

Chin-Fang Chang, Atikul Islam, Pei-Fen Liu, Jun-Han Zhan, Pin Ju Chueh

Open access · greenAbstract read

In one paragraph

Article in American journal of cancer research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it

0cells of the map it votes in

14citing papers in PubMed, 1 pooled it

2.5field-weighted citation impact, top 11% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.

Capsaicin: A Two-Decade Systematic Review of Global Research Output and Recent Advances Against Human Cancer.Frontiers in oncology · 2022
Pooled it
Protective effect of capsaicin on AKT1 and MAPK1 expression in the liver of mice (Open veterinary journal · 2025
Article
Ecto-NOX Disulfide-Thiol Exchanger 2 (ENOX2/tNOX) Is a Potential Prognostic Marker in Primary Malignant Melanoma and May Serve as a Therapeutic Target.International journal of molecular sciences · 2024
Article
Unlocking the potential of capsaicin in oral health (Review).Biomedical reports · 2024
Review
Targeting histone deacetylases in head and neck squamous cell carcinoma: molecular mechanisms and therapeutic targets.Journal of translational medicine · 2024
Review
Water-soluble 4-(dimethylaminomethyl)heliomycin exerts greater antitumor effects than parental heliomycin by targeting the tNOX-SIRT1 axis and apoptosis in oral cancer cells.eLife · 2024
Article
Antioxidants (Basel, Switzerland) · 2024
Article
Capsaicinoids and Their Effects on Cancer: The "Double-Edged Sword" Postulate from the Molecular Scale.Cells · 2023
Review
Implications of altered sirtuins in metabolic regulation and oral cancer.PeerJ · 2023
Review
Capsaicin decreases fecundity in the Asian malaria vector Anopheles stephensi by inhibiting the target of rapamycin signaling pathway.Parasites & vectors · 2022
Article
Bis(chloroacetamidino)-Derived Heteroarene-Fused Anthraquinones Bind to and Cause Proteasomal Degradation of tNOX, Leading to c-Flip Downregulation and Apoptosis in Oral Cancer Cells.Cancers · 2022
Article
How Should the Worldwide Knowledge of Traditional Cancer Healing Be Integrated with Herbs and Mushrooms into Modern Molecular Pharmacology?Pharmaceuticals (Basel, Switzerland) · 2022
Review
Identification and validation of seven RNA binding protein genes as a prognostic signature in oral cavity squamous cell carcinoma.Bioengineered · 2021
Article
Capsaicin exerts therapeutic effects by targeting tNOX-SIRT1 axis and augmenting ROS-dependent autophagy in melanoma cancer cells.American journal of cancer research · 2021
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Chin-Fang ChangDepartment of Otolaryngology, Head and Neck Surgery, Jen-Ai Hospital Taichung 41265, Taiwan.

Atikul IslamInstitute of Biomedical Sciences, National Chung Hsing University 145 Xingda Road, Taichung 40227, Taiwan.

Pei-Fen LiuDepartment of Food Science and Biotechnology, National Chung Hsing University 145 Xingda Road, South District, Taichung 40227, Taiwan.

Jun-Han ZhanBachelor Program of Biotechnology, National Chung Hsing University 145 Xingda Road, Taichung 40227, Taiwan.

Pin Ju ChuehInstitute of Biomedical Sciences, National Chung Hsing University 145 Xingda Road, Taichung 40227, Taiwan.

National Chung Hsing University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite the progress that has been made in diagnosing and treating oral cancers, they continue to have a poor prognosis, with a 5-year overall survival rate of approximately 50%. We have intensively studied the anticancer properties of capsaicin (a burning constituent of chili pepper), mainly focusing on its apoptotic properties. Here, we investigated the interplay between apoptosis and autophagy in capsaicin-treated oral cancer cells with either functional or mutant p53. Cytotoxicity was determined by cell impedance measurements and WST-1 assays, and cell death was analyzed by flow cytometry. The interaction between capsaicin and tumor-associated NADH oxidase (tNOX, ENOX2) was studied by cellular thermal shift assay (CETSA) and isothermal dose-response fingerprint curves (ITDRF

Indexed as

Apoptosisautophagycapsaicinoral cancersilent mating type information regulation 1 (Sirtuin 1, SIRT1)tumor-associated NADH oxidase (tNOX, ENOX2)

Identifiers

PMID33163267

PMCPMC7642647

OpenAlexW3106140194

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.