ArticleFrontiers in microbiology2020
Application of Short Pre-enrichment, and Double Chemistry Real-Time PCR, Combining Fluorescent Probes and an Intercalating Dye, for Same-Day Detection and Confirmation of
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Design and evaluation of a streamlined enrichment method for faster co-detection of enteric bacterial pathogens in natural soils and environmental samples.medRxiv : the preprint server for health sciences · 2026Article
- Article
- Detection of theOpen veterinary journal · 2024Article
- Development of Shortened Enrichment Methods for Detection ofACS food science & technology · 2023Article
- Study on the mechanism of inhibiting patulin production by fengycin.Open life sciences · 2022Article
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Authors and funding
5 authors.
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Abstract
Molecular methods, particularly those based on real-time PCR (qPCR), have become a popular approach to detect pathogens in food samples. This technique may take advantage of hydrolysis fluorescent probes for increased specificity. Even though suitable, this approach loses the capacity of performing result confirmation by melt curve analysis. In the current study, we developed an alternative approach, combining fluorescent probes along with an intercalating dye (SYBR Green) in order to simultaneously detect, and confirm the result, of two foodborne pathogens (
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