Evidence map›Paper›PMID 33162754›Full record

ArticleInternational journal of nanomedicine2020

Combating Intracellular Pathogens with Nanohybrid-Facilitated Antibiotic Delivery.

Rajendran J C Bose, Nagendran Tharmalingam, Yonghyun Choi, Thiagarajan Madheswaran, Ramasamy Paulmurugan, Jason R McCarthy, Soo-Hong Lee, Hansoo Park

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of nanomedicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 3 countries.

Rajendran J C Bose *School of Integrative Engineering, Chung-Ang University, Seoul, South Korea.ORCID 0000-0001-7592-3908
Nagendran Tharmalingam *Infectious Diseases Division, Warren Alpert Medical School of Brown University, Rhode Island Hospital, Providence, RI 02903, USA.ORCID 0000-0001-6107-3754
Yonghyun ChoiSchool of Integrative Engineering, Chung-Ang University, Seoul, South Korea.
Thiagarajan MadheswaranDepartment of Pharmaceutical Technology, School of Pharmacy, International Medical University, Kuala Lumpur 57000, Malaysia.ORCID 0000-0001-5049-0659
Ramasamy PaulmuruganMolecular Imaging Program at Stanford (MIPS), Department of Radiology, School of Medicine, Stanford University, Stanford, CA 94305-5427, USA.ORCID 0000-0001-7155-4738
Jason R McCarthyMasonic Medical Research Institute, Utica, NY, USA.ORCID 0000-0002-5302-2030
Soo-Hong LeeDepartment of Medical Biotechnology, Dongguk University, Seoul, Gyeonggi-do, South Korea.
Hansoo ParkSchool of Integrative Engineering, Chung-Ang University, Seoul, South Korea.
Chung-Ang University · KRMasonic Medical Research Laboratory · USBrown University · USDongguk University · KRIMU University · MYStanford University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLipid polymer hybrid nanoparticles (LPHNPs) have been widely investigated in drug and gene delivery as well as in medical imaging. A knowledge of lipid-based surface engineering and its effects on how the physicochemical properties of LPHNPs affect the cell-nanoparticle interactions, and consequently how it influences the cytological response, is in high demand.

methodsHerein, we have engineered antibiotic-loaded (doxycycline or vancomycin) LPHNPs with cationic and zwitterionic lipids and examined the effects on their physicochemical characteristics (size and charge), antibiotic entrapment efficiency, and the in vitro intracellular bacterial killing efficiency against

resultsThe incorporation of cationic or zwitterionic lipids in the LPHNP formulation resulted in a size reduction in LPHNPs formulations and shifted the surface charge of bare NPs towards positive or neutral values. Also observed were influences on the drug incorporation efficiency and modulation of the drug release from the biodegradable polymeric core. The therapeutic efficacy of LPHNPs loaded with vancomycin was improved as its minimum inhibitory concentration (MIC) (2 µg/mL) versus free vancomycin (4 µg/mL). Importantly, our results show a direct relationship between the cationic surface nature of LPHNPs and its intracellular bacterial killing efficiency as the cationic doxycycline or vancomycin loaded LPHNPs reduced 4 or 3 log CFU respectively versus the untreated controls.

conclusionIn our study, modulation of surface charge in the nanomaterial formulation increased macrophage uptake and intracellular bacterial killing efficiency of LPHNPs loaded with antibiotics, suggesting alternate way for optimizing their use in biomedical applications.

Indexed as

Drug Delivery SystemsAnimalsAnti-Bacterial AgentsCell LineCell SurvivalDrug LiberationIntracellular SpaceLipidsMacrophagesMiceMicrobial Sensitivity TestsMycobacterium smegmatisNanoparticlesParticle SizePolymersStaphylococcus aureusAnti-Bacterial AgentsLipidsPolymersVancomycindoxycyclineintracellular bacterial infectionMycobacterium smegmatisnanohybridsStaphylococcus aureusvancomycin

Identifiers

PMID33162754
PMCPMC7642590
OpenAlexW3097370753

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.