ArticleThe Journal of cell biology1987
In vivo coassembly of a divergent beta-tubulin subunit (c beta 6) into microtubules of different function.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 65 citations in OpenAlex.
- Regulation of Tubulin Gene Expression: From Isotype Identity to Functional Specialization.Frontiers in cell and developmental biology · 2022Review
- Characterization of a human βV-tubulin antibody and expression of this isotype in normal and malignant human tissue.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Class III β-tubulin counteracts the ability of paclitaxel to inhibit cell migration.Oncotarget · 2011Article
- Class V β-tubulin alters dynamic instability and stimulates microtubule detachment from centrosomes.Molecular biology of the cell · 2011Article
- Megakaryocyte lineage-specific class VI β-tubulin suppresses microtubule dynamics, fragments microtubules, and blocks cell division.Cytoskeleton (Hoboken, N.J.) · 2011Article
- The beta isotypes of tubulin in neuronal differentiation.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Article
- A ubiquitous beta-tubulin disrupts microtubule assembly and inhibits cell proliferation.Molecular biology of the cell · 2004Article
- Selective expression of beta tubulin isotypes in gerbil vestibular sensory epithelia and neurons.Journal of the Association for Research in Otolaryngology : JARO · 2003Article
- Gamma-tubulin can both nucleate microtubule assembly and self-assemble into novel tubular structures in mammalian cells.The Journal of cell biology · 1995Article
- Either alpha-tubulin isogene product is sufficient for microtubule function during all stages of growth and differentiation in Aspergillus nidulans.Molecular and cellular biology · 1993Article
- A nucleotide substitution in one of the beta-tubulin genes of Trichoderma viride confers resistance to the antimitotic drug methyl benzimidazole-2-yl-carbamate.Molecular & general genetics : MGG · 1993Article
- Two alpha-tubulin genes of Aspergillus nidulans encode divergent proteins.Molecular & general genetics : MGG · 1991Article
- Tubulin Isotypes in Rye Roots Are Altered during Cold Acclimation.Plant physiology · 1990Article
- Copolymerization of two distinct tubulin isotypes during microtubule assembly in vitro.The Journal of cell biology · 1990Article
- Differential utilization of beta-tubulin isotypes in differentiating neurites.The Journal of cell biology · 1989Article
- Diversity among beta-tubulins: a carboxy-terminal domain of yeast beta-tubulin is not essential in vivo.Molecular and cellular biology · 1988Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
alpha- and beta-Tubulin are encoded in vertebrate genomes by a family of approximately 6-7 functional genes whose polypeptide products differ in amino acid sequence. In the chicken, one beta-tubulin isotype (c beta 6) has previously been found to be expressed only in thrombocytes and erythroid cells, where it is assembled into a circumferential ring of marginal band microtubules. In light of its unique in vivo utilization and its divergent assembly properties in vitro, we used DNA transfection to test whether this isotype could be assembled in vivo into microtubules of divergent functions. Using an antibody specific to c beta 6, we have found that upon transfection this polypeptide is freely coassembled into an extensive array of interphase cytoplasmic microtubules and into astral and pole-to-chromosome or pole-to-pole microtubules during mitosis. Further, examination of developing chicken erythrocytes reveals that both beta-tubulins that are expressed in these cells (c beta 6 and c beta 3) are found as co-polymers of the two isoforms. These results, in conjunction with efforts that have localized various other beta-tubulin isotypes, demonstrate that to the resolution limit afforded by light microscopy in vivo microtubules in vertebrates are random copolymers of available isotypes. Although these findings are consistent with functional interchangeability of beta-tubulin isotypes, we have also found that in vivo microtubules enriched in c beta 3 polypeptides are more sensitive to cold depolymerization than those enriched in c beta 6. This differential quantitative utilization of the two endogenous isotypes documents that some in vivo functional differences between isotypes do exist.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.