Evidence map›Paper›PMID 33159703›Full record

ArticleJournal of fish diseases2021

Method for cryopreservation of Paramoeba perurans.

Christiane Tröße, Mats Kindt, Steffen Blindheim, Linda Andersen, Are Nylund

Abstract read
In one paragraph

Article in Journal of fish diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Christiane TrößeDepartment of Biological Sciences, University of Bergen, Bergen, Norway.ORCID https://orcid.org/0000-0002-7259-8211
Mats KindtDepartment of Biological Sciences, University of Bergen, Bergen, Norway.
Steffen BlindheimThe Aquatic and Industrial Laboratory (ILAB), Bergen, Norway.
Linda AndersenThe Aquatic and Industrial Laboratory (ILAB), Bergen, Norway.
Are NylundDepartment of Biological Sciences, University of Bergen, Bergen, Norway.

Funding

Fiskeri - og havbruksnaeringens forskningsfond 901053
6 · The paper itself

Abstract

Paramoeba perurans causes amoebic gill disease (AGD), which is a major problem in aquaculture worldwide. The parasite can be cultured in vitro, but to this date, no method for long-term storage of the clones exists. In this study, we describe a method for cryopreservation of Paramoeba perurans. The method was successfully employed on four out the five clones we tested. The thawing success rate, that is the percentage of successfully thawed vials relative to the total number of vials that were thawed, differed for the clones and ranged from 25% to 100%. The age of the clones seemed to have a negative impact on the ability to survive cryopreservation.

Indexed as

AmoebozoaAmebiasisCryopreservationFish DiseasesNorwaycryopreservationNeoparamoebaParamoebaperurans

Identifiers

PMID33159703
PMCPMC8246932

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.