Evidence map›Paper›PMID 33159262›Full record

ArticleActa parasitologica2021

The Effect of Naja naja oxiana Snake Venom Against Leishmania tropica Confirmed by Advanced Assays.

Iraj Sharifi, Fatemeh Tabatabaie, Saeideh Nikpour, Mahshid Mostafavi, Razieh Tavakoli Oliaee, Fatemeh Sharifi, Zahra Babaei, Elham Jafari, Ehsan Salarkia, Delavar Shahbazzadeh

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Article in Acta parasitologica, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Iraj SharifiLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Fatemeh TabatabaieDepartment of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. tabatabaie.f98@gmail.com.ORCID http://orcid.org/0000-0002-3169-7758
Saeideh NikpourDepartment of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. saeidehnikpour@yahoo.com.
Mahshid MostafaviLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Razieh Tavakoli OliaeeLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Fatemeh SharifiResearch Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran.
Zahra BabaeiLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Elham JafariPathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Ehsan SalarkiaLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Delavar ShahbazzadehLaboratory of Venom and Biotherapeutics Molecules, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Kerman University of Medical Sciences · IRIran University of Medical Sciences · IRPasteur Institute of Iran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe aim of this study was to explore the activity of Naja naja oxiana venom on Leishmania tropica and its modes of action.

methodsDifferent fractions of Naja naja oxiana venom (NNOV) were prepared and characterized by high-performance liquid chromatography. The superior component, fraction k (FK) was selected. The activity of the fraction was assessed using advanced assays.

resultsInterleukin (IL)-12, TNF-α and iNOS gene expression as the indicators of Th1 significantly increased. In contrast, the level of IL-10, as the marker of T helper 2 substantially decreased (p < 0.001). Reactive oxygen species (ROS) detection showed a significant increase (p < 0.001) after treatment with different concentrations of NNOV-FK, unlike arginase (L-ARG) activity which showed a significant reduction (p < 0.001). The NNOV-FK showed significant lethal activity on the L. tropica stages.

conclusionThe findings demonstrated that NNOV-FK represented a strong leishmanicidal activity on L. tropica stages. The major modes of NNOV-FK action are multidimensional, which perceives the induction of a synergistic response and upregulation of the immune-modulatory role towards Th1 response against L. tropica stages as well as apoptotic and anti-metabolic action as a model drug to generate ROS, block the polyamine synthesis and lead to parasite death.

Indexed as

Leishmania tropicaNaja najaAnimalsBiological AssayElapid VenomsSnake VenomsElapid VenomsSnake VenomsLeishmania tropicaLeishmanicidal effectMechanism of actionSnake venom fractions

Identifiers

PMID33159262
OpenAlexW3095557961

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.