Evidence map›Paper›PMID 33159251›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithelial Cells.

Yoonjoo K Lee, Kristen L Segars, Vickery Trinkaus-Randall

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Yoonjoo K LeeDepartments of Pharmacology and Biochemistry, Boston University School of Medicine, Boston, MA, USA.
Kristen L SegarsDepartments of Pharmacology and Biochemistry, Boston University School of Medicine, Boston, MA, USA.
Vickery Trinkaus-RandallDepartments of Pharmacology and Biochemistry, Boston University School of Medicine, Boston, MA, USA. vickery@bu.edu.
Boston University · US

Funding

Molecular Mechanisms of corneal wound repairR01EY006000 · NEI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI TRINKAUS-RANDALL, VICKERY E · 1986 to 2018
$6.8M
Molecular Mechanisms of corneal wound repairR01EY032079 · NEI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI TRINKAUS-RANDALL, VICKERY E · 2021 to 2023
$1.2M
Calcium oscillations and cell motility with corneal injuryR21EY029097 · NEI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI TRINKAUS-RANDALL, VICKERY E · 2019 to 2020
$454k
The Role of Sustained Calcium Oscillations in the Wound Healing ResponseF30EY028001 · NEI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI LEE, YOONJOO KATHERINE · 2017 to 2020
$199k
NEI NIH HHS F30 EY028001NEI NIH HHS R01 EY006000NEI NIH HHS R21 EY029097
6 · The paper itself

Abstract

Chemical indicators are used to study calcium signaling events in the context of live cell imaging. Fluo-3 AM, Fluo-4 AM, and Cal-520 AM are three commonly used fluorescent indicators derived from the calcium chelator BAPTA. Here we describe sample protocols that detail how these indicators are used in in vitro and ex vivo experiments to analyze the role of calcium mobilizations in cell-cell communication and coordinated cellular motility in the context of wound healing.

Indexed as

CalciumCell CommunicationEpithelium, CornealHumansCalciumCalcium mobilizationCell-cell communicationConfocal imagingEx vivo in situ analysisImage analysisLive cell imaging

Identifiers

PMID33159251
PMCPMC8787922
OpenAlexW3095502895

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.