ArticleCommunications biology2020
Cell culture-based karyotyping of orectolobiform sharks for chromosome-scale genome analysis.
Article in Communications biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Improved genome assembly of whale shark, the world's biggest fish: revealing intragenomic heterogeneity in molecular evolution.GigaScience · 2026Article
- The inbred newt genome unveils molecular mechanisms of behavior, development, and regeneration in urodele amphibians.iScience · 2025Article
- Chromosome-level genome assembly and Oligo-FISH confirmation of chromosome models for the Tasmanian snow skink Carinascincus ocellatus (Scincidae, Eugongylinae).BMC biology · 2025Article
- Chromosome-Level Assembly and Annotation of the Grey Reef Shark (Carcharhinus amblyrhynchos) Genome.Genome biology and evolution · 2025Article
- First successful intra-uterine artificial insemination in a cownose ray (Rhinoptera javanica) using chilled semen.The Journal of veterinary medical science · 2025Article
- Sharks and rays have the oldest vertebrate sex chromosome with unique sex determination mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cell bank system, establishment, and application in the virus research, diagnosis, and biopharmaceutical industries.Biotechnology notes (Amsterdam, Netherlands) · 2025Review
- Comparative genomics illuminates karyotype and sex chromosome evolution of sharks.Cell genomics · 2024Article
- Primary Cell Culture as a Model System for Evolutionary Molecular Physiology.International journal of molecular sciences · 2024Review
- Cell Identification, Characterization, and Documentation for Use in the Production of Biological Products.Archives of Razi Institute · 2024Review
- Shark genome size evolution and its relationship with cellular, life-history, ecological, and diversity traits.Scientific reports · 2024Article
- Multiple Transitions between Y Chromosome and Autosome in Tago's Brown Frog Species Complex.Genes · 2024Article
- A novel report on the emerging and zoonotic neurotropic fungus Trichosporon japonicum in the brain tissue of the endangered Brazilian guitarfish (Pseudobatos horkelii) off the southeastern coast of Brazil.BMC microbiology · 2023Article
- Low mutation rate in epaulette sharks is consistent with a slow rate of evolution in sharks.Nature communications · 2023Article
- Elasmobranch genome sequencing reveals evolutionary trends of vertebrate karyotype organization.Genome research · 2023Article
- Squalomix: shark and ray genome analysis consortium and its data sharing platform.F1000Research · 2022Article
- Technical considerations in Hi-C scaffolding and evaluation of chromosome-scale genome assemblies.Molecular ecology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Karyotyping, traditionally performed using cytogenetic techniques, is indispensable for validating genome assemblies whose sequence lengths can be scaled up to chromosome sizes using modern methods. Karyotype reports of chondrichthyans are scarce because of the difficulty in cell culture. Here, we focused on carpet shark species and the culture conditions for fibroblasts and lymphocytes. The utility of the cultured cells enabled the high-fidelity characterization of their karyotypes, namely 2n = 102 for the whale shark (Rhincodon typus) and zebra shark (Stegostoma fasciatum), and 2n = 106 for the brownbanded bamboo shark (Chiloscyllium punctatum) and whitespotted bamboo shark (C. plagiosum). We identified heteromorphic XX/XY sex chromosomes for the two latter species and demonstrated the first-ever fluorescence in situ hybridization of shark chromosomes prepared from cultured cells. Our protocols are applicable to diverse chondrichthyan species and will deepen the understanding of early vertebrate evolution at the molecular level.
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