ArticleNature communications2020
Client proximity enhancement inside cellular membrane-less compartments governed by client-compartment interactions.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Advances in engineering and applications of synthetic phase-separated membraneless organelles in biotechnology.Synthetic and systems biotechnology · 2026Review
- Biomolecular condensates in plant immunity.Cell host & microbe · 2025Review
- Optical Control of Microtubule Accumulation and Dispersion by Tau-Derived Peptide-Fused Photoresponsive Protein.JACS Au · 2025Article
- Heterogeneous Slowdown of Dynamics in the Condensate of an Intrinsically Disordered Protein.The journal of physical chemistry letters · 2024Article
- Protein Condensate Atlas from predictive models of heteromolecular condensate composition.Nature communications · 2024Article
- Article
- Construction of multiphasic membraneless organelles towards spontaneous spatial segregation and directional flow of biochemical reactions.Chemical science · 2023Article
- Synthetic protein condensates for cellular and metabolic engineering.Nature chemical biology · 2022Review
- Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences.Biochemistry · 2022Article
- Reaction-Diffusion Patterning of DNA-Based Artificial Cells.Journal of the American Chemical Society · 2022Article
- Recent trends in studies of biomolecular phase separation.BMB reports · 2022Article
- <i>Drosophila</i> insulator proteins exhibit in vivo liquid-liquid phase separation properties.Life science alliance · 2022Article
- Conformational Dynamics of Intrinsically Disordered Proteins Regulate Biomolecular Condensate Chemistry.Chemical reviews · 2022Review
- Determinants for intrinsically disordered protein recruitment into phase-separated protein condensates.Chemical science · 2022Article
- Current Understanding of Molecular Phase Separation in Chromosomes.International journal of molecular sciences · 2021Review
- Designer membraneless organelles sequester native factors for control of cell behavior.Nature chemical biology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane-less organelles or compartments are considered to be dynamic reaction centers for spatiotemporal control of diverse cellular processes in eukaryotic cells. Although their formation mechanisms have been steadily elucidated via the classical concept of liquid-liquid phase separation, biomolecular behaviors such as protein interactions inside these liquid compartments have been largely unexplored. Here we report quantitative measurements of changes in protein interactions for the proteins recruited into membrane-less compartments (termed client proteins) in living cells. Under a wide range of phase separation conditions, protein interaction signals were vastly increased only inside compartments, indicating greatly enhanced proximity between recruited client proteins. By employing an in vitro phase separation model, we discovered that the operational proximity of clients (measured from client-client interactions) could be over 16 times higher than the expected proximity from actual client concentrations inside compartments. We propose that two aspects should be considered when explaining client proximity enhancement by phase separation compartmentalization: (1) clients are selectively recruited into compartments, leading to concentration enrichment, and more importantly, (2) recruited clients are further localized around compartment-forming scaffold protein networks, which results in even higher client proximity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.