Evidence map›Paper›PMID 33158200›Full record

ArticleViruses2020

Portable Rabies Virus Sequencing in Canine Rabies Endemic Countries Using the Oxford Nanopore MinION.

Crystal M Gigante, Gowri Yale, Rene Edgar Condori, Niceta Cunha Costa, Nguyen Van Long, Phan Quang Minh, Vo Dinh Chuong, Nguyen Dang Tho, Nguyen Tat Thanh, Nguyen Xuan Thin and 19 more

Abstract read
In one paragraph

Article in Viruses, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

  1. Article
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  11. Nanopore sequencing in veterinary medicine: from concepts to clinical applications.Frontiers in cellular and infection microbiology · 2025
    Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
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  19. Genomic epidemiology on the move.Nature reviews. Microbiology · 2023
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

29 authors.

Crystal M GigantePoxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.ORCID 0000-0002-2169-7156
Gowri YaleMission Rabies, Tonca, Panjim, Goa 403001, India.ORCID 0000-0002-8953-7167
Rene Edgar CondoriPoxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.ORCID 0000-0001-9812-7963
Niceta Cunha CostaDisease Investigation Unit, Directorate of Animal Health and Veterinary Services, Patto, Panjim, Goa 403001, India.
Nguyen Van LongVietnam Department of Animal Health, Hanoi 100000, Vietnam.
Phan Quang MinhVietnam Department of Animal Health, Hanoi 100000, Vietnam.
Vo Dinh ChuongVietnam Department of Animal Health, Hanoi 100000, Vietnam.
Nguyen Dang ThoNational Center for Veterinary Diseases, Hanoi 100000, Vietnam.
Nguyen Tat ThanhSub-Department of Animal Health, Phú Thọ Province 35000, Vietnam.
Nguyen Xuan ThinSub-Department of Animal Health, Phú Thọ Province 35000, Vietnam.
Nguyen Thi Hong HanhSub-Department of Animal Health, Phú Thọ Province 35000, Vietnam.
Gati WamburaCenter for Global Health Research, Kenya Medical Research Institute, Nairobi 00100, Kenya.
Frederick AdeCenter for Global Health Research, Kenya Medical Research Institute, Nairobi 00100, Kenya.
Oscar MitoCenter for Global Health Research, Kenya Medical Research Institute, Nairobi 00100, Kenya.
Veronicah ChuchuCenter for Global Health Research, Kenya Medical Research Institute, Nairobi 00100, Kenya.
Mathew MuturiZoonotic Disease Unit, Ministry of Health, Ministry of Agriculture, Livestock and Fisheries, Nairobi 00100, Kenya.ORCID 0000-0003-2110-8099
Athman MwatondoZoonotic Disease Unit, Ministry of Health, Ministry of Agriculture, Livestock and Fisheries, Nairobi 00100, Kenya.
Katie HampsonInstitute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Glasgow G12 8QQ, UK.ORCID 0000-0001-5392-6884
Samuel M ThumbiCenter for Global Health Research, Kenya Medical Research Institute, Nairobi 00100, Kenya.ORCID 0000-0002-5754-0556
Byron G ThomaeMinistry of Agriculture Livestock and Food, Guatemala City 01013, Guatemala.
Victor Hugo de PazNational Health Laboratory, MSPAS, Villa Nueva 01064, Guatemala.
Sergio MenesesNational Health Laboratory, MSPAS, Villa Nueva 01064, Guatemala.
Peninah MunyuaDivision of Global Health Protection, Centers for Disease Control, Nairobi 00100, Kenya.
David MoranUniversity del Valle de Guatemala, Guatemala City 01015, Guatemala.
Loren CadenaDivision of Global Health Protection, Centers for Disease Control, Guatemala City 01001, Guatemala.
Andrew GibsonThe Roslin Institute and The Royal (Dick) School of Veterinary Studies, Division of Genetics and Genomics, The University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK.ORCID 0000-0002-4641-2583
Ryan M WallacePoxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.
Emily G PieracciPoxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.ORCID 0000-0002-7735-6051
Yu LiPoxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA.

Funding

Medical Research Council MR/R025649/1Wellcome Trust 110330/Z/15/ZWellcome Trust 207569/Z/17/Z
6 · The paper itself

Abstract

As countries with endemic canine rabies progress towards elimination by 2030, it will become necessary to employ techniques to help plan, monitor, and confirm canine rabies elimination. Sequencing can provide critical information to inform control and vaccination strategies by identifying genetically distinct virus variants that may have different host reservoir species or geographic distributions. However, many rabies testing laboratories lack the resources or expertise for sequencing, especially in remote or rural areas where human rabies deaths are highest. We developed a low-cost, high throughput rabies virus sequencing method using the Oxford Nanopore MinION portable sequencer. A total of 259 sequences were generated from diverse rabies virus isolates in public health laboratories lacking rabies virus sequencing capacity in Guatemala, India, Kenya, and Vietnam. Phylogenetic analysis provided valuable insight into rabies virus diversity and distribution in these countries and identified a new rabies virus lineage in Kenya, the first published canine rabies virus sequence from Guatemala, evidence of rabies spread across an international border in Vietnam, and importation of a rabid dog into a state working to become rabies-free in India. Taken together, our evaluation highlights the MinION's potential for low-cost, high volume sequencing of pathogens in locations with limited resources.

Indexed as

AnimalsDiagnostic EquipmentDog DiseasesDogsEndemic DiseasesGuatemalaHumansIndiaKenyaNanoporesPhylogenyPublic HealthRabiesRabies virusSequence Analysis, DNAVietnamcanine rabies eliminationlyssavirusMinIONnanoporeportable sequencingrabies

Identifiers

PMID33158200
PMCPMC7694271

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.