ArticleCryobiology2021
Rapid quantification of multi-cryoprotectant toxicity using an automated liquid handling method.
Article in Cryobiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- A High-Throughput Platform for Measuring and Predicting Vitrification Behavior in Multicomponent Aqueous Solutions.ACS applied materials & interfaces · 2026Article
- Lessons From Drug Discovery for Cryoprotective Agent Design: An AI-Oriented Perspective.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A high throughput platform for measuring and predicting vitrification behavior in multicomponent aqueous solutions.bioRxiv : the preprint server for biology · 2026Article
- Optimizing South African giraffe Epididymal sperm preservation techniques".Veterinary and animal science · 2026Article
- High-throughput evaluation of cryoprotective agents for mixture effects that reduce toxicity.Cryobiology · 2025Article
- Higher glass transition temperatures reduce thermal stress cracking in aqueous solutions relevant to cryopreservation.Scientific reports · 2025Article
- A size-dependent ideal solution model for liquid-solid phase equilibria prediction in aqueous organic solutions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.Scientific reports · 2025Article
- Article
- High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.bioRxiv : the preprint server for biology · 2024Article
- Evaluation of heat transfer in porous scaffolds under cryogenic treatment: a numerical study.Medical & biological engineering & computing · 2023Article
- Zebrafish as a high throughput model for organ preservation and transplantation research.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- Accelerating cryoprotectant delivery using vacuum infiltration.Cryobiology · 2023Article
- Sound waves for solving the problem of recrystallization in cryopreservation.Scientific reports · 2023Article
- Technologies for Vitrification Based Cryopreservation.Bioengineering (Basel, Switzerland) · 2023Review
- A comprehensive review and update on human fertility cryopreservation methods and tools.Frontiers in veterinary science · 2023Review
- Article
- Chemical approaches to cryopreservation.Nature reviews. Chemistry · 2022Review
- Vitrification Solutions for Plant Cryopreservation: Modification and Properties.Plants (Basel, Switzerland) · 2021Review
- General tissue mass transfer model for cryopreservation applications.Biophysical journal · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Cryopreservation in a vitrified state has vast potential for long-term storage of tissues and organs that may be damaged by ice formation. However, the toxicity imparted by the high concentration of cryoprotectants (CPAs) required to vitrify these specimens remains a hurdle. To address this challenge, we previously developed a mathematical approach to design less toxic CPA equilibration methods based on the minimization of a toxicity cost function. This approach was used to design improved methods for equilibration of bovine pulmonary artery endothelial cells (BPAEC) with glycerol. To fully capitalize on the toxicity cost function approach, it is critical to describe the toxicity kinetics of additional CPAs, including multi-CPA mixtures that are commonly used for vitrification. In this work, we used automated liquid handling to characterize the toxicity kinetics of five of the most common CPAs (glycerol, dimethyl sulfoxide (DMSO), propylene glycol, ethylene glycol, and formamide), along with their binary and ternary mixtures for BPAEC. In doing so, we developed experimental methods that can be used to determine toxicity kinetics more quickly and accurately. Our results highlight some common CPA toxicity trends, including the relatively low toxicity of ethylene glycol and a general increase in toxicity as the CPA concentration increases. Our results also suggest potential new approaches to reduce toxicity, including a surprising toxicity neutralization effect of glycerol on formamide. In the future, this dataset will serve as the basis to expand our CPA toxicity model, enabling application of the toxicity cost function approach to vitrification solutions containing multiple CPAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.