Evidence map›Paper›PMID 33155431›Full record

ArticleFEBS open bio2021

miR-34a-5p up-regulates the IL-1β/COX2/PGE2 inflammation pathway and induces the release of CGRP via inhibition of SIRT1 in rat trigeminal ganglion neurons.

Hui Zhang, Xue-Mei Zhang, Dan-Dan Zong, Xiao-Ying Ji, Hua Jiang, Feng-Zheng Zhang, Sheng-Dong He

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.

  1. The Role of MicroRNA in Migraine: A Systemic Literature Review.Cellular and molecular neurobiology · 2023
    Pooled it
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  6. Inhibition of the METTL3/mThe journal of headache and pain · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Hui ZhangThe Second Affiliated Hospital of Guilin Medical University, China.
Xue-Mei ZhangCollege of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, China.
Dan-Dan ZongThe Second Xiangya Hospital, Central South University, Changsha, China.
Xiao-Ying JiThe Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, China.
Hua JiangCollege of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, China.
Feng-Zheng ZhangCollege of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, China.
Sheng-Dong HeThe Second Affiliated Hospital of Guilin Medical University, China.ORCID 0000-0002-6530-3315
Southwest Medical University · CNGuilin Medical University · CNCentral South University · CNSun Yat-sen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Migraine is a debilitating neurological condition, with a global prevalence rate of 10.68% in men and 18.79% in women. Elucidation of the molecular mechanisms underlying migraines is of great importance for improving the quality of life of patients. The release of the neuropeptide calcitonin gene-related peptide (CGRP) from trigeminal nerve terminals is involved in the pathogenesis of migraine. Recent studies have shown that up-regulation of miR-34a-5p expression is associated with acute migraine attacks. Here, we investigated whether alteration of the expression of miR-34a-5p induces the release of the vasoactive peptide CGRP. We isolated primary rat trigeminal ganglion neurons and performed gain- and loss-of-function assays to alter the expression level of miR-34a-5p. Down-regulation of miR-34a-5p inhibited the expression of interleukin-1β (IL-1β)/cyclooxygenase 2 (COX2)/prostaglandin E2 (PGE2), decreased IL-1β, PGE2 and CGRP release, and up-regulated the expression of silencing information regulator 1 (SIRT1) in trigeminal ganglion, whereas overexpression of miR-34a-5p enhanced the expression of IL-1β/COX2/PGE2, increased the release of IL-1β, PGE2 and CGRP, and decreased the expression of SIRT1 in trigeminal ganglion. In addition, overexpression of miR-34a-5p induced apoptosis in primary rat trigeminal neurons. In summary, these findings suggest that miR-34a-5p up-regulates the IL-1β/COX2/PGE2 inflammation pathway, induces apoptosis and enhances release of CGRP via inhibition of SIRT1 expression in trigeminal ganglion neurons; thus, miR-34a-5p may have potential as a therapeutic target for the treatment of migraine.

Indexed as

AnimalsAnimals, NewbornApoptosisCalcitonin Gene-Related PeptideCells, CulturedCyclooxygenase 2DinoprostoneDisease Models, AnimalDown-RegulationHumansInflammationInterleukin-1betaMicroRNAsMigraine DisordersNeuronsPrimary Cell CultureCalcitonin Gene-Related PeptideCyclooxygenase 2DinoprostoneIL1B protein, ratInterleukin-1betaMicroRNAsMIRN34 microRNA, ratPtgs2 protein, ratSirt1 protein, ratSirtuin 1CGRPCOX2migrainemiR-34a-5pPGE2SIRT1

Identifiers

PMID33155431
PMCPMC7780114
OpenAlexW3097575190

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.