Evidence map›Paper›PMID 33155064›Full record

ArticleProtoplasma2021

Effects of the protein GCP4 on gametophyte development in Arabidopsis thaliana.

Dongjing Ma, Lin Gao, Rong Han

Abstract read
PubMed Publisher
In one paragraph

Article in Protoplasma, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Dongjing MaHigher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, 041000, Shanxi, People's Republic of China.
Lin GaoHigher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, 041000, Shanxi, People's Republic of China.
Rong HanHigher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, 041000, Shanxi, People's Republic of China. hhwrsl@163.com.
Shanxi Normal University · CN

Funding

2018 Graduate Science and Technology Innovation of Shanxi Normal University 010530122019 Innovation Project of Postgraduate Education of Shanxi Province 2019BY090
6 · The paper itself

Abstract

γ-Tubulin complex protein 4 (GCP4, encoded by AT3G53760) participates in microtubule (MT) nucleation in Arabidopsis thaliana, affecting the MT nucleation angles in cortical MTs, and the formation of the spindle and phragmoplasts during mitosis. Here, we report that GCP4 plays a critical role in gametophyte development. The results indicate that the gcp4 mutant caused by T-DNA insertion may express an aberrant gene product interfering with normal GCP4 expression, ultimately leading to the formation of desiccated ovules and aborted seeds. An analysis of transmission efficiency (TE) indicated that female gametophytes were more impaired in development than male gametophytes, and so observation and analysis of gametophyte defects were conducted. Complementation lines obtained by the native promoter and GCP4-coded CDS gene sequence fused with GFP reduced the numbers of lethal phenotypes of the gcp4 mutant. The localization of GCP4 in the gametophyte was detected in cytoplasm around nuclei and in vicinity of plasma membrane of pollen grains, and also detected in full cytoplasm and around the nuclei of ovules in complementation line. Thus, it was established that GCP4 influences the functionality of gametophytes during gametophyte development.

Indexed as

ArabidopsisArabidopsis ProteinsPollenArabidopsis ProteinsArabidopsis thalianaAT3G53760Gametophyte defectsMicrotubulesγ-Tubulin complex protein 4

Identifiers

PMID33155064
OpenAlexW3096023128

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.