ArticleScientific reports2020
Placenta-derived macaque trophoblast stem cells: differentiation to syncytiotrophoblasts and extravillous trophoblasts reveals phenotypic reprogramming.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Advantages, limitations, and innovative considerations for established and emerging models of human placental syncytiotrophoblast.Human reproduction update · 2026Review
- Comparative analysis of rhesus macaque and human placental organoids highlights evolutionary differences in placentation.Developmental cell · 2026Article
- Article
- Effects of an aryl hydrocarbon receptor ligand on human trophoblast cell development.Human reproduction (Oxford, England) · 2025Article
- In vitro differentiation of macaque extravillous trophoblasts in a low oxygen environment.Placenta · 2025Article
- Aryl Hydrocarbon Receptor Activation Drives 2-Methoxy Estradiol Secretion in Human Trophoblast Stem Cell Development.bioRxiv : the preprint server for biology · 2025Article
- Wnt signaling activation confers a syncytiotrophoblast progenitor state on trophoblast stem cells of cynomolgus monkey†.Biology of reproduction · 2024Article
- Trophoblast Organoids: Capturing the Complexity of Early Placental Development In Vitro.Organoids · 2024Article
- Conserved and divergent features of trophoblast stem cells.Journal of molecular endocrinology · 2024Review
- Animal models of postpartum hemorrhage.Lab animal · 2024Review
- Zika virus co-opts microRNA networks to persist in placental niches detected by spatial transcriptomics.American journal of obstetrics and gynecology · 2024Article
- Vertical transmission of African-lineage Zika virus through the fetal membranes in a rhesus macaque (Macaca mulatta) model.PLoS pathogens · 2023Article
- Regulation of human trophoblast gene expression by endogenous retroviruses.Nature structural & molecular biology · 2023Article
- Zika virus impacts extracellular vesicle composition and cellular gene expression in macaque early gestation trophoblasts.Scientific reports · 2022Article
- Efficient derivation of human trophoblast stem cells from primed pluripotent stem cells.Science advances · 2021Article
- Transcriptomic analysis of primate placentas and novel rhesus trophoblast cell lines informs investigations of human placentation.BMC biology · 2021Article
- The promise of placental extracellular vesicles: models and challenges for diagnosing placental dysfunction in utero†.Biology of reproduction · 2021Review
- Similarities and differences in placental development between humans and cynomolgus monkeys.Reproductive medicine and biologyReview
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
Nonhuman primates are excellent models for studying human placentation as experimental manipulations in vitro can be translated to in vivo pregnancy. Our objective was to develop macaque trophoblast stem cells (TSCs) as an in vitro platform for future assessment of primate trophoblast development and function. Macaque TSC lines were generated by isolating first and second trimester placental villous cytotrophoblasts followed by culture in TSC medium to maintain cellular proliferation. TSCs grew as mononuclear colonies, whereas upon induction of syncytiotrophoblast (ST) differentiation multinuclear structures appeared, indicative of syncytium formation. Chorionic gonadotropin secretion was > 4000-fold higher in ST culture media compared to TSC media. The secretion of chorionic gonadotropin by TSC-derived ST reflects a reprogramming of macaque TSCs to an earlier pregnancy phenotype. Characteristic trophoblast hallmarks were defined in TSCs and ST including expression of C19MC miRNAs and the macaque placental nonclassical MHC class I molecule, Mamu-AG. Extravillous trophoblasts (EVTs) were derived that express macaque EVT markers Mamu-AG and CD56, and also secrete high levels of MMP2. Our analyses of macaque TSCs suggests that these cells represent a proliferative, self-renewing population capable of differentiating to STs and EVTs in vitro thereby establishing an experimental model of primate placentation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.