ArticleScientific reports2020
Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 54 citations in OpenAlex.
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- RNAi-mediated knockdown of the poultry red mite cathepsin D-1 impacts haemoglobin digestion.Parasites & vectors · 2026Article
- Recent advances in understanding of the mechanisms of RNA interference in insects.Insect molecular biology · 2025Review
- Target gene selection for sprayable dsRNA-based biopesticide against Tetranychus urticae Koch (Acari: Tetranychidae).Pest management science · 2025Article
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- The role of polyplexes in developing a green sustainable approach in agriculture.RSC advances · 2022Review
- Lipopolysaccharide detection by the innate immune system may be an uncommon defence strategy used in nature.Open biology · 2022Review
- Localized efficacy of environmental RNAi in Tetranychus urticae.Scientific reports · 2022Article
- β-Cyanoalanine synthase protects mites against Arabidopsis defenses.Plant physiology · 2022Article
- Trunk injection delivery of dsRNA for RNAi-based pest control in apple trees.Pest management science · 2022Article
- Hydroxynitrile lyase defends Arabidopsis against Tetranychus urticae.Plant physiology · 2022Article
- RNA meets toxicology: efficacy indicators from the experimental design of RNAi studies for insect pest management.Pest management science · 2022Review
- Article
- Molecular characterization, expression, and function of Vitellogenin genes in Phytoseiulus persimilis.Experimental & applied acarology · 2022Article
- Innate and adaptive resistance to RNAi: a major challenge and hurdle to the development of double stranded RNA-based pesticides.3 Biotech · 2021Review
- RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges.Plants (Basel, Switzerland) · 2021Article
- A Leaf-Mimicking Method for Oral Delivery of Bioactive Substances Into Sucking Arthropod Herbivores.Frontiers in plant science · 2020Article
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16 authors at 4 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comprehensive understanding of pleiotropic roles of RNAi machinery highlighted the conserved chromosomal functions of RNA interference. The consequences of the evolutionary variation in the core RNAi pathway genes are mostly unknown, but may lead to the species-specific functions associated with gene silencing. The two-spotted spider mite, Tetranychus urticae, is a major polyphagous chelicerate pest capable of feeding on over 1100 plant species and developing resistance to pesticides used for its control. A well annotated genome, susceptibility to RNAi and economic importance, make T. urticae an excellent candidate for development of an RNAi protocol that enables high-throughput genetic screens and RNAi-based pest control. Here, we show that the length of the exogenous dsRNA critically determines its processivity and ability to induce RNAi in vivo. A combination of the long dsRNAs and the use of dye to trace the ingestion of dsRNA enabled the identification of genes involved in membrane transport and 26S proteasome degradation as sensitive RNAi targets. Our data demonstrate that environmental RNAi can be an efficient reverse genetics and pest control tool in T. urticae. In addition, the species-specific properties together with the variation in the components of the RNAi machinery make T. urticae a potent experimental system to study the evolution of RNAi pathways.
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