Evidence map›Paper›PMID 33153478›Full record

ArticleMolecular cancer2020

circCUL2 regulates gastric cancer malignant transformation and cisplatin resistance by modulating autophagy activation via miR-142-3p/ROCK2.

Lei Peng, Huaiming Sang, Shuchun Wei, Yuanyuan Li, Duochen Jin, Xudong Zhu, Xuan Li, Yini Dang, Guoxin Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cancer, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 149 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
149citing papers in PubMed, 2 pooled it
12.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

149 citing papers in PubMed, 2 syntheses or guidelines pooled it, 237 citations in OpenAlex.

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89 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Lei PengDepartment of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Huaiming SangDepartment of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Shuchun WeiDepartment of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Yuanyuan LiDepartment of endocrinology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Duochen JinDepartment of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Xudong ZhuDepartment of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Xuan LiDepartment of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Yini DangDepartment of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Guoxin ZhangDepartment of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China. guoxinz@njmu.edu.cn.
Jiangsu Province Hospital · CNNanjing Medical University · CNSecond Affiliated Hospital of Nanjing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCircular RNAs (circRNAs) are a class of noncoding RNAs (ncRNAs) and can modulate gene expression by binding to miRNAs; further, circRNAs have been shown to participate in several pathological processes. However, the expression and biological function of circCUL2 in gastric cancer (GC) remains largely unknown.

methodscircRNA microarrays and quantitative real-time PCR (qRT-PCR) were used to identify differentially expressed circRNAs in GC tissues and cell lines. circCUL2 knockdown and overexpression were performed to indicate the functional role of circCUL2 in vitro and in vivo. The expression and regulation of circCUL2, miR-142-3p and ROCK2 were evaluated using fluorescence in situ hybridization (FISH), dual-luciferase assays, RNA pull-down assays, RNA immunoprecipitation (RIP) and rescue experiments. Furthermore, the regulation of cisplatin sensitivity and autophagy by circCUL2/miR-142-3p/ROCK2 was demonstrated by cellular apoptosis assays, western blot, immunofluorescence and transmission electron microscopy analyses.

resultsThe level of circCUL2, which is stable and cytoplasmically localized, was significantly reduced in GC tissues and cells. Overexpressed circCUL2 inhibited malignant transformation in vitro and tumorigenicity in vivo. In the AGS and SGC-7901 cell lines, circCUL2 sponged miR-142-3p to regulate ROCK2, thus modulating tumor progression. Furthermore, in the AGS/DDP and SGC-7901/DDP cell lines, circCUL2 regulated cisplatin sensitivity through miR-142-3p/ROCK2-mediated autophagy activation.

conclusioncircCUL2 may function as a tumor suppressor and regulator of cisplatin sensitivity through miR-142-3p/ROCK2-mediated autophagy activation, which could be a key mechanism and therapeutic target for GC.

Indexed as

AutophagyDrug Resistance, NeoplasmAnimalsAntineoplastic AgentsApoptosisBiomarkers, TumorCell MovementCell ProliferationCell Transformation, NeoplasticCisplatinCullin ProteinsFemaleGene Expression Regulation, NeoplasticHumansMaleMiceAntineoplastic AgentsBiomarkers, TumorCisplatinCUL2 protein, humanCullin ProteinsMicroRNAsMIRN142 microRNA, humanrho-Associated KinasesRNA, CircularROCK2 protein, humanAutophagycircCUL2Cisplatin resistanceGastric cancermiR-142-3pROCK2

Identifiers

PMID33153478
PMCPMC7643398
OpenAlexW3097885939

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.