Evidence map›Paper›PMID 33153359›Full record

ArticleNucleus (Austin, Tex.)2020

Automated 3D bio-imaging analysis of nuclear organization by NucleusJ 2.0.

Tristan Dubos, Axel Poulet, Céline Gonthier-Gueret, Guillaume Mougeot, Emmanuel Vanrobays, Yanru Li, Sylvie Tutois, Emilie Pery, Frédéric Chausse, Aline V Probst and 2 more

Open access · goldAbstract readVideo-Audio Media
In one paragraph

Article in Nucleus (Austin, Tex.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
5.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 31 citations in OpenAlex.

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  14. Methods of In Situ Quantitative Root Biology.Plants (Basel, Switzerland) · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 4 countries.

Tristan DubosGReD, CNRS, INSERM, Université Clermont Auvergne , Clermont-Ferrand, France58.ORCID 0000-0002-4265-2379
Axel PouletDepartment of Molecular, Cellular & Developmental Biology, Yale University , New Haven, CT, USA.
Céline Gonthier-GueretNeurodol, INSERM, Université Clermont Auvergne , Clermont-Ferrand, France.ORCID 0000-0002-4449-559X
Guillaume MougeotGReD, CNRS, INSERM, Université Clermont Auvergne , Clermont-Ferrand, France58.ORCID 0000-0003-3576-7300
Emmanuel VanrobaysGReD, CNRS, INSERM, Université Clermont Auvergne , Clermont-Ferrand, France58.ORCID 0000-0002-3209-6743
Yanru LiDepartment of Plant and Microbial Biology, Zürich-Basel Plant Science Center, University of Zürich , Zürich, Switzerland.
Sylvie TutoisGReD, CNRS, INSERM, Université Clermont Auvergne , Clermont-Ferrand, France58.ORCID 0000-0003-3429-2470
Emilie PeryInstitut Pascal, Université Clermont Auvergne , Clermont-Ferrand, France.ORCID 0000-0001-6198-9973
Frédéric ChausseInstitut Pascal, Université Clermont Auvergne , Clermont-Ferrand, France.ORCID 0000-0001-7794-1587
Aline V ProbstGReD, CNRS, INSERM, Université Clermont Auvergne , Clermont-Ferrand, France58.ORCID 0000-0001-9534-8058
Christophe TatoutGReD, CNRS, INSERM, Université Clermont Auvergne , Clermont-Ferrand, France58.ORCID 0000-0001-5215-2338
Sophie DessetGReD, CNRS, INSERM, Université Clermont Auvergne , Clermont-Ferrand, France58.ORCID 0000-0002-4897-4977
Centre National de la Recherche Scientifique · FRInstitut Pascal · FRInserm · FROxford Brookes University · GBUniversity of Zurich · CHYale University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

NucleusJ 1.0, an ImageJ plugin, is a useful tool to analyze nuclear morphology and chromatin organization in plant and animal cells. NucleusJ 2.0 is a new release of NucleusJ, in which image processing is achieved more quickly using a command-lineuser interface. Starting with large collection of 3D nuclei, segmentation can be performed by the previously developed Otsu-modified method or by a new 3D gift-wrapping method, taking better account of nuclear indentations and unstained nucleoli. These two complementary methods are compared for their accuracy by using three types of datasets available to the community at

Indexed as

Cell NucleolusDatabases, FactualImaging, Three-DimensionalSoftware3D DNA FISHimage analysisnuclear organizationplant nucleusThree-dimensional microscopy imaging

Identifiers

PMID33153359
PMCPMC7714466
OpenAlexW3096074721

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.