ArticleMaterials (Basel, Switzerland)2020
Label-Free DNA Biosensor Using Modified Reduced Graphene Oxide Platform as a DNA Methylation Assay.
Article in Materials (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 32 citations in OpenAlex.
- Label-Free Electrochemical Biosensors: An Updated Perspective Focused on Genosensing, Multiplexing, and Commercial Potential.Biosensors · 2026Review
- Recent Advances in the Electrochemical Biosensing of DNA Methylation.International journal of molecular sciences · 2025Review
- Solid Phase Oligo-DNA Extraction from Complex Medium Using an Aminated Graphene/Nitrocellulose Membrane Hybrid.Biomolecules · 2024Article
- HpaII-assisted and linear amplification-enhanced isothermal exponential amplification fluorescent strategy for rapid and sensitive detection of DNA methyltransferase activity.Analytical and bioanalytical chemistry · 2023Article
- Current Perspectives in Graphene Oxide-Based Electrochemical Biosensors for Cancer Diagnostics.Biosensors · 2022Review
- Rapid and sensitive detection of box turtles using an electrochemical DNA biosensor based on a gold/graphene nanocomposite.Beilstein journal of nanotechnology · 2022Article
- A Fluorescence Kinetic-Based Aptasensor Employing Stilbene Isomerization for Detection of Thrombin.Materials (Basel, Switzerland) · 2021Article
- DNA Methylation in Solid Tumors: Functions and Methods of Detection.International journal of molecular sciences · 2021Review
- Recent Advances in Electrochemical Sensors for the Detection of Biomolecules and Whole Cells.Biomedicines · 2020Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
This work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)-gold (AuNPs), silver (AgNPs), and copper (CuNPs)-was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA-1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5'-CCGG-3') islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U·mL
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Registered trials
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