ArticleJournal of innate immunity2021
RNA Sequencing Reveals Diverse Functions of Amniotic Fluid Neutrophils and Monocytes/Macrophages in Intra-Amniotic Infection.
Article in Journal of innate immunity, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- The amniotic fluid cell-free transcriptome in spontaneous preterm labor.Scientific reports · 2021Trial
- Immune cellular homeostasis and its breakdown at the maternal-fetal interface.Trends in immunology · 2026Review
- Executive summary: Workshop on preterm birth, January 28-29, 2025, co-sponsored by the American College of Obstetricians and Gynecologists and the Eunice Kennedy Shriver National Institute of Child Health and Human Development.Pregnancy (Hoboken, N.J.) · 2025Article
- Decoding the amniotic membrane transcriptome during equine ascending placentitis.Scientific reports · 2025Article
- The maternal-fetal interface at single-cell resolution: uncovering the cellular anatomy of the placenta and decidua.American journal of obstetrics and gynecology · 2025Review
- Characterizing neuroinflammation and identifying prenatal diagnostic markers for neural tube defects through integrated multi-omics analysis.Journal of translational medicine · 2024Article
- Evidence for the participation of CHCHD2/MNRR1, a mitochondrial protein, in spontaneous labor at term and in preterm labor with intra-amniotic infection.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2023Article
- Thymic stromal lymphopoietin participates in the host response to intra-amniotic inflammation leading to preterm labor and birth.Human immunology · 2023Article
- BAFF and APRIL counterregulate susceptibility to inflammation-induced preterm birth.Cell reports · 2023Article
- Intra-amniotic inflammation in the mid-trimester of pregnancy is a risk factor for neuropsychological disorders in childhood.Journal of perinatal medicine · 2023Article
- A single-cell atlas of murine reproductive tissues during preterm labor.Cell reports · 2023Article
- Omics approaches: interactions at the maternal-fetal interface and origins of child health and disease.Pediatric research · 2023Review
- Placental transcriptomic signatures of spontaneous preterm birth.American journal of obstetrics and gynecology · 2023Article
- RNA-Seq of amniotic fluid cell-free RNA: a discovery phase study of the pathophysiology of congenital cytomegalovirus infection.American journal of obstetrics and gynecology · 2022Article
- The Role of Neutrophils in Pregnancy, Term and Preterm Labour.Life (Basel, Switzerland) · 2022Review
- Article
- The amniotic fluid proteome predicts imminent preterm delivery in asymptomatic women with a short cervix.Scientific reports · 2022Article
- The immunobiology of preterm labor and birth: intra-amniotic inflammation or breakdown of maternal-fetal homeostasis.Reproduction (Cambridge, England) · 2022Review
- Pregnancy tailors endotoxin-induced monocyte and neutrophil responses in the maternal circulation.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2022Article
- IL-22 Plays a Dual Role in the Amniotic Cavity: Tissue Injury and Host Defense against Microbes in Preterm Labor.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
Intra-amniotic infection, the invasion of microbes into the amniotic cavity resulting in inflammation, is a clinical condition that can lead to adverse pregnancy outcomes for the mother and fetus as well as severe long-term neonatal morbidities. Despite much research focused on the consequences of intra-amniotic infection, there remains little knowledge about the innate immune cells that respond to invading microbes. We performed RNA-seq of sorted amniotic fluid neutrophils and monocytes/macrophages from women with intra-amniotic infection to determine the transcriptomic differences between these innate immune cells. Further, we sought to identify specific transcriptomic pathways that were significantly altered by the maternal or fetal origin of amniotic fluid neutrophils and monocytes/macrophages, the presence of a severe fetal inflammatory response, and pregnancy outcome (i.e., preterm or term delivery). We show that significant transcriptomic differences exist between amniotic fluid neutrophils and monocytes/macrophages from women with intra-amniotic infection, indicating the distinct roles these cells play. The transcriptome of amniotic fluid immune cells varies based on their maternal or fetal origin, and the significant transcriptomic differences between fetal and maternal monocytes/macrophages imply that those of fetal origin exhibit impaired functions. Notably, transcriptomic changes in amniotic fluid monocytes/macrophages suggest that these immune cells collaborate with neutrophils in the trafficking of fetal leukocytes throughout the umbilical cord (i.e., funisitis). Finally, amniotic fluid neutrophils and monocytes/macrophages from preterm deliveries display enhanced transcriptional activity compared to those from term deliveries, highlighting the protective role of these cells during this vulnerable period. Collectively, these findings demonstrate the underlying complexity of local innate immune responses in women with intra-amniotic infection and provide new insights into the functions of neutrophils and monocytes/macrophages in the amniotic cavity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.