Evidence map›Paper›PMID 33152409›Full record

ArticleJournal of virological methods2021

Development of a swine RNA polymerase I driven Influenza reverse genetics system for the rescue of type A and B Influenza viruses.

Brittany Seibert, Matthew Angel, C Joaquin Caceres, Troy Sutton, Ayush Kumar, Lucas Ferreri, Stivalis Cardenas-Garcia, Ginger Geiger, Daniela Rajao, Daniel R Perez

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Brittany SeibertDepartment of Population Health, Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, United States.
Matthew AngelCellular Biology Section, Laboratory of Viral Diseases NIAID, NIH, Bethesda, MD, United States.
C Joaquin CaceresDepartment of Population Health, Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, United States.
Troy SuttonDepartment of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA, United States.
Ayush KumarDepartment of Population Health, Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, United States.
Lucas FerreriDepartment of Population Health, Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, United States.
Stivalis Cardenas-GarciaDepartment of Population Health, Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, United States.
Ginger GeigerDepartment of Population Health, Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, United States.
Daniela RajaoDepartment of Population Health, Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, United States.
Daniel R PerezDepartment of Population Health, Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, United States. Electronic address: dperez1@uga.edu.
University of Georgia · USNational Institute of Allergy and Infectious Diseases · USPennsylvania State University · US

Funding

Flu-IGIP: Live attenuated influenza virus vaccines with improved stimulation of IgA responsesR21AI146448 · NIAID · UNIVERSITY OF GEORGIA · PI PEREZ, DANIEL R · 2019 to 2020
$433k
NIAID NIH HHS HHSN272201400008CNIAID NIH HHS R21 AI146448
6 · The paper itself

Abstract

Influenza viruses are among the most significant pathogens of humans and animals. Reverse genetics allows for the study of molecular attributes that modulate virus host range, virulence and transmission. The most common reverse genetics methods use bi-directional vectors containing a host RNA polymerase (pol) I promoter to produce virus-like RNAs and a host RNA pol II promoter to direct the synthesis of viral proteins. Given the species-dependency of the pol I promoter and virus-host interactions that influence replication of animal-origin influenza viruses in human-derived cells, we explored the potential of using the swine RNA pol I promoter (spol1) in a bi-directional vector for rescuing type A and B influenza viruses (IAV and IBV, respectively) in swine and human cells. The spol1-based bi-directional plasmid vector led to efficient rescue of IAVs of different origins (human, swine, and avian) as well as IBV in both swine- and human-origin tissue culture cells. In addition, virus rescue was successful using a recombinant bacmid containing all eight segments of a swine origin IAV. In conclusion, the spol1-based reverse genetics system is a new platform to study influenza viruses and produce swine influenza vaccines with increased transfection efficiency.

Indexed as

Herpesvirus 1, CercopithecineInfluenza, HumanInfluenza VaccinesOrthomyxoviridaeAnimalsHumansReverse GeneticsRNA Polymerase ISwineInfluenza VaccinesRNA Polymerase IHuman cellsInfluenza virusReverse geneticsSwine cellsSwine RNA polymerase I

Identifiers

PMID33152409
PMCPMC8103788
OpenAlexW3094840796

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.