ArticlePLoS neglected tropical diseases2020
Evaluation of the diagnostic accuracy of lateral flow devices as a tool to diagnose rabies in post-mortem animals.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- High sensitivity of the Bionote Anigen Rapid Rabies Antigen Test in detection of diverse rabies virus variants suggests utility for global rabies control.Journal of clinical microbiology · 2026Article
- LFD implementation uncovers hidden rabies burden: Pre- and post-implementation analysis and comparison with non-implementation sites in the Philippines.One health (Amsterdam, Netherlands) · 2025Article
- Validation and multi-site deployment of a lyophilized qRT-PCR reagent for the molecular diagnosis of avian influenza and rabies in Sub-Saharan African regions.Journal of clinical microbiology · 2025Article
- The Challenge of Lyssavirus Infections in Domestic and Other Animals: A Mix of Virological Confusion, Consternation, Chagrin, and Curiosity.Pathogens (Basel, Switzerland) · 2025Review
- Incidence and prevalence of rabies virus infections in tested humans and animals in Asia: A systematic review and meta-analysis study.One health (Amsterdam, Netherlands) · 2025Review
- Evaluation of lateral flow devices for rabies diagnosis in decomposed animal brain samples.Tropical medicine and health · 2025Article
- Evaluating Rabies Test Accuracy: A Systematic Review and Meta-Analysis of Human and Canine Diagnostic Methods.Diagnostics (Basel, Switzerland) · 2025Review
- First report on dog bite epidemiology and Rabies diagnosis in stray dogs: a one health study from Puducherry.Frontiers in veterinary science · 2025Article
- Investigating the Impact That Diagnostic Screening with Lateral Flow Devices Had on the Rabies Surveillance Program in Zanzibar, Tanzania.Microorganisms · 2024Article
- Evaluation of lateral flow devices for postmortem rabies diagnosis in animals in the Philippines: a multicenter study.Journal of clinical microbiology · 2023Article
- Using Integrated Bite Case Management to estimate the burden of rabies and evaluate surveillance in Oriental Mindoro, Philippines.One health & implementation research · 2023Article
- Molecular Analysis of Rabies Virus Using RNA Extracted from Used Lateral Flow Devices.Journal of clinical microbiology · 2023Article
- Evaluation of a real-time mobile PCR device (PCR 1100) for the detection of the rabies gene in field samples.Tropical medicine and health · 2023Article
- United Against Rabies Forum: The first 2 years.Frontiers in public health · 2023Review
- A call to accelerate an end to human rabies deaths.Lancet (London, England) · 2022Review
- Lateral flow devices for samples collected by straw sampling method for postmortem canine rabies diagnosis.PLoS neglected tropical diseases · 2021Article
- Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Background and descriptive features of rabies-suspected animals in Central Luzon, Philippines.Tropical medicine and health · 2021Article
- Capacity Building Efforts for Rabies Diagnosis in Resource-Limited Countries in Sub-Saharan Africa: A Case Report of the Central Veterinary Laboratory in Benin (Parakou).Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
13 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Implementation of lateral flow devices (LFDs) for rabies antigen detection is expected to improve surveillance through the efficient detection of rabid animals in resource-limited settings; however, the use of LFDs for diagnosis remains controversial because some commercially available kits show low sensitivity. Therefore, we compared the diagnostic efficacy of three LFDs (ADTEC, Bionote, and Elabscience kits) paralleled with the direct fluorescent antibody test (dFAT) using fresh samples and investigated the diagnostic accuracies. To do so, we evaluated rabies-suspected samples submitted to the Regional Animal Disease Diagnostic Laboratory III, Philippines. Furthermore, we conducted real-time RT-PCR and sequencing to measure the accuracy of field laboratory diagnosis. The total number of animals submitted during this study period was 184 cases, including negative control samples. Of these, 53.9% (84 cases) were positive in the dFAT. Dogs were the most common rabies-suspected animal (n = 135). The sensitivities of the ADTEC and Bionote kits were 0.88 (74 cases) and 0.95 (80 cases), respectively. The specificity of both kits was 1.00 (100 cases). Furthermore, the sensitivity and specificity of the ADTEC kit after directly homogenizing the samples in assay buffer without dilution in phosphate-buffered saline (ADTEC kit DM) were 0.94 (79 cases) and 1.00 (100 cases), respectively. By contrast, there were no positive results using the Elabscience kit among all dFAT-positive samples. The sensitivity and specificity of LFDs make these tests highly feasible if properly used. Therefore, LFD tests can be used to strengthen the surveillance of rabies-infected animals in endemic and resource-limited settings.
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