Evidence map›Paper›PMID 33149993›Full record

ArticleBiomedical optics express2020

Au nanostar nanoparticle as a bio-imaging agent and its detection and visualization in biosystems.

E Perevedentseva, N Ali, Y-C Lin, A Karmenyan, C-C Chang, O Bibikova, I Skovorodkin, R Prunskaite-Hyyryläinen, S J Vainio, M Kinnunen and 1 more

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Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
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  4. Antibiotics (Basel, Switzerland) · 2023
    Article
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  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

E PerevedentsevaDepartment of Physics, National Dong Hwa University, Hualien, 97401, Taiwan.
N AliBiocenter Oulu, Infotech Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Borealis Biobank of Northern Finland, Oulu University Hospital, P.O. Box 8000 FI-90014 Oulu, Finland.
Y-C LinDepartment of Physics, National Dong Hwa University, Hualien, 97401, Taiwan.
A KarmenyanDepartment of Physics, National Dong Hwa University, Hualien, 97401, Taiwan.
C-C ChangDepartment of Physics, National Dong Hwa University, Hualien, 97401, Taiwan.
O BibikovaBiocenter Oulu, Infotech Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Borealis Biobank of Northern Finland, Oulu University Hospital, P.O. Box 8000 FI-90014 Oulu, Finland.
I SkovorodkinBiocenter Oulu, Infotech Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Borealis Biobank of Northern Finland, Oulu University Hospital, P.O. Box 8000 FI-90014 Oulu, Finland.
R Prunskaite-HyyryläinenBiocenter Oulu, Infotech Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Borealis Biobank of Northern Finland, Oulu University Hospital, P.O. Box 8000 FI-90014 Oulu, Finland.
S J VainioBiocenter Oulu, Infotech Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Borealis Biobank of Northern Finland, Oulu University Hospital, P.O. Box 8000 FI-90014 Oulu, Finland.
M KinnunenFaculty of Information Technology and Electrical Engineering, University of Oulu, P.O. Box 8000 FI-90014 Oulu, Finland.ORCID https://orcid.org/0000-0002-8338-2520
C-L ChengDepartment of Physics, National Dong Hwa University, Hualien, 97401, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present work, we report the imaging of Au nanostars nanoparticles (AuNSt) and their multifunctional applications in biomedical research and theranostics applications. Their optical and spectroscopic properties are considered for the multimodal imaging purpose. The AuNSt are prepared by the seed-meditated method and characterized for use as an agent for bio-imaging. To demonstrate imaging with AuNSt, penetration and localization in different biological models such as cancer cell culture (A549 lung carcinoma cell), 3D tissue model (multicellular tumor spheroid on the base of human oral squamous carcinoma cell, SAS) and murine skin tissue are studied. AuNSt were visualized using fluorescence lifetime imaging (FLIM) at two-photon excitation with a pulse duration 140 fs, repetition rate 80 MHz and 780 nm wavelength femtosecond laser. Strong emission of AuNSt at two-photon excitation in the near infrared range and fluorescence lifetime less than 0.5 ns were observed. It allows using AuNSt as a fluorescent marker at two-photon fluorescence microscopy and lifetime imaging (FLIM). It was shown that AuNSt can be observed inside a thick sample (tissue and its model). This is the first demonstration using AuNSt as an imaging agent for FLIM at two-photon excitation in biosystems. Increased scattering of near-infrared light upon excitation of AuNSt surface plasmon oscillation was also observed and rendered using a possible contrast agent for optical coherence tomography (OCT). AuNSt detection in a biological system using FLIM is compared with OCT on the model of AuNSt penetrating into animal skin. The AuNSt application for multimodal imaging is discussed.

Identifiers

PMID33149993
PMCPMC7587281

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