ArticleBiomedical optics express2020
X-ray Zernike phase contrast tomography: 3D ROI visualization of mm-sized mice organ tissues down to sub-cellular components.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- In situ ptychographic x-ray nanotomography of temperature-controlled crystallization processes.Nature communications · 2026Article
- The Use of Synchrotron Radiation in the Medical Sciences.Photon science · 2026Article
- Radiation-dose effects in correlative X-ray/cryo-electron microscopy of frozen-hydrated biological samples.Acta crystallographica. Section D, Structural biology · 2026Article
- Design and implementation of a climate chamber for moisture sensitive nanotomography of biological samples.Journal of synchrotron radiation · 2025Article
- Experimental comparison of X-ray ptychographic and holographic nanotomography of metal-stained neuronal tissue.Optics express · 2025Article
- Deep learning to overcome Zernike phase-contrast nanoCT artifacts for automated micro-nano porosity segmentation in bone.Journal of synchrotron radiation · 2024Article
- Hard X-ray full-field nanoimaging using a direct photon-counting detector.Journal of synchrotron radiation · 2023Article
- Nondestructive cellular-level 3D observation of mouse kidney using laboratory-based X-ray microscopy with paraffin-mediated contrast enhancement.Scientific reports · 2022Article
- A multiscale X-ray phase-contrast tomography dataset of a whole human left lung.Scientific data · 2022Article
- Machine learning denoising of high-resolution X-ray nanotomography data.Journal of synchrotron radiation · 2022Article
- Evaluating the morphology of the degradation layer of pure magnesiumBioactive materials · 2021Article
- 3D Spatial Distribution of Nanoparticles in Mice Brain Metastases by X-ray Phase-Contrast Tomography.Frontiers in oncology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thanks to its non-invasive nature, X-ray phase contrast tomography is a very versatile imaging tool for biomedical studies. In contrast, histology is a well-established method, though having its limitations: it requires extensive sample preparation and it is quite time consuming. Therefore, the development of nano-imaging techniques for studying anatomic details at the cellular level is gaining more and more importance. In this article, full field transmission X-ray nanotomography is used in combination with Zernike phase contrast to image millimeter sized unstained tissue samples at high spatial resolution. The regions of interest (ROI) scans of different tissues were obtained from mouse kidney, spleen and mammalian carcinoma. Thanks to the relatively large field of view and effective pixel sizes down to 36 nm, this 3D approach enabled the visualization of the specific morphology of each tissue type without staining or complex sample preparation. As a proof of concept technique, we show that the high-quality images even permitted the 3D segmentation of multiple structures down to a sub-cellular level. Using stitching techniques, volumes larger than the field of view are accessible. This method can lead to a deeper understanding of the organs' nano-anatomy, filling the resolution gap between histology and transmission electron microscopy.
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Registered trials
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