ArticleScientific reports2020
Systems evaluation reveals novel transporter YohJK renders 3-hydroxypropionate tolerance in Escherichia coli.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
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- Transcriptional and post-transcriptional mechanisms modulate cyclopropane fatty acid synthase through small RNAs inJournal of bacteriology · 2024Article
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- A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century.Microbial biotechnology · 2023Review
- Characterisation of aneLife · 2023Article
- EngineeringFrontiers in bioengineering and biotechnology · 2023Article
- Benchmarking recombinant Pichia pastoris for 3-hydroxypropionic acid production from glycerol.Microbial biotechnology · 2021Article
- Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria.World journal of microbiology & biotechnology · 2021Review
- Global RNA profiles show target selectivity and physiological effects of peptide-delivered antisense antibiotics.Nucleic acids research · 2021Article
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Authors and funding
6 authors.
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Abstract
Previously, we have reported that 3-hydroxypropionate (3-HP) tolerance in Escherichia coli W is improved by deletion of yieP, a less-studied transcription factor. Here, through systems analyses along with physiological and functional studies, we suggest that the yieP deletion improves 3-HP tolerance by upregulation of yohJK, encoding putative 3-HP transporter(s). The tolerance improvement by yieP deletion was highly specific to 3-HP, among various C2-C4 organic acids. Mapping of YieP binding sites (ChIP-exo) coupled with transcriptomic profiling (RNA-seq) advocated seven potential genes/operons for further functional analysis. Among them, the yohJK operon, encoding for novel transmembrane proteins, was the most responsible for the improved 3-HP tolerance; deletion of yohJK reduced 3-HP tolerance regardless of yieP deletion, and their subsequent complementation fully restored the tolerance in both the wild-type and yieP deletion mutant. When determined by 3-HP-responsive biosensor, a drastic reduction of intracellular 3-HP was observed upon yieP deletion or yohJK overexpression, suggesting that yohJK encodes for novel 3-HP exporter(s).
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