ArticleThe ISME journal2021
Plasmid- and strain-specific factors drive variation in ESBL-plasmid spread in vitro and in vivo.
Article in The ISME journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 125 citations in OpenAlex.
- Microbiome ecology and antibiotic treatment failure: resistance spread, microbial competition and community engineering.Microbial genomics · 2026Review
- Role of extended-spectrum β-lactamase (ESBL) genes in the dissemination of β-lactam resistance within aquatic environments: mechanisms, persistence, and one health implications.World journal of microbiology & biotechnology · 2026Review
- Plasmid-Host Networks Reveal Persistent Mobile Elements Across Wastewater Treatment.Nature communications · 2026Article
- Modelling the role of the microbiome in antimicrobial resistance across scales.Nature microbiology · 2026Review
- Targeting bacterial phosphotransferase system to prevent the dissemination of antibiotic resistance genes.Acta pharmaceutica Sinica. B · 2026Article
- Article
- Integrative analysis of fitness and metabolic effects of a large multidrug-resistant plasmid inMicrobial genomics · 2026Article
- Antimicrobial resistance, conjugative plasmids and pathogenicity in wastewater and freshwater Escherichia spp. in Stockholm, Sweden.npj antimicrobials and resistance · 2026Article
- Fast hospital discharge rates blur within-hospital 'transmission footprint' in bacterial genomes, as showcased with Staphylococcus aureus.PLoS computational biology · 2026Article
- Conjugation frequency of ESBL- and pAmpC- E. coli in broiler chickens in vivo and in vitro.BMC microbiology · 2026Article
- Transfer potential of F-like plasmids inISME communications · 2026Article
- Differential gut transmission of IncP plasmid clades involving hypervirulent Klebsiella pneumoniae reveals plasmid-specific ecological adaptation.Nature communications · 2025Article
- The occurrences ofIranian journal of microbiology · 2025Article
- Multi-layered ecological interactions determine growth of clinical antibiotic-resistant strains within human microbiomes.Nature communications · 2025Article
- Antimicrobial resistance and molecular characterization of ESBL-producing Enterobacterales from Parirenyatwa Hospital wastewater in Harare.JAC-antimicrobial resistance · 2025Article
- A multi-isolate genomic approach identifies diverseMicrobial genomics · 2025Article
- Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli in Pet Birds of Bangladesh.Veterinary medicine and science · 2025Article
- Fluid flow generates bacterial conjugation hot spots by increasing the rate of shear-driven cell-cell encounters.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Molecular detection of blaSHV gene in multidrug resistance ofOpen veterinary journal · 2025Article
- The role of bacterial metabolism in human gut colonization.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Review
3 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Horizontal gene transfer, mediated by conjugative plasmids, is a major driver of the global rise of antibiotic resistance. However, the relative contributions of factors that underlie the spread of plasmids and their roles in conjugation in vivo are unclear. To address this, we investigated the spread of clinical Extended Spectrum Beta-Lactamase (ESBL)-producing plasmids in the absence of antibiotics in vitro and in the mouse intestine. We hypothesised that plasmid properties would be the primary determinants of plasmid spread and that bacterial strain identity would also contribute. We found clinical Escherichia coli strains natively associated with ESBL-plasmids conjugated to three distinct E. coli strains and one Salmonella enterica serovar Typhimurium strain. Final transconjugant frequencies varied across plasmid, donor, and recipient combinations, with qualitative consistency when comparing transfer in vitro and in vivo in mice. In both environments, transconjugant frequencies for these natural strains and plasmids covaried with the presence/absence of transfer genes on ESBL-plasmids and were affected by plasmid incompatibility. By moving ESBL-plasmids out of their native hosts, we showed that donor and recipient strains also modulated transconjugant frequencies. This suggests that plasmid spread in the complex gut environment of animals and humans can be predicted based on in vitro testing and genetic data.
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