ArticleCell communication and signaling : CCS2020
Proteomics identifies differences in fibrotic potential of extracellular vesicles from human tendon and muscle fibroblasts.
Article in Cell communication and signaling : CCS, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Effect of human myoblasts on tenogenic progression in 2D and 3D culture models.Journal of anatomy · 2026Article
- Spectroscopic fingerprinting of extracellular vesicles from diverse cellular origins by ATR-FTIR for vibrational biomarkers of vector-host interactions.Scientific reports · 2026Article
- Sarcopenia beyond muscle: endocrine-metabolic regulation and inter-tissue crosstalk across the muscle-tendon unit.Frontiers in endocrinology · 2026Review
- Myoblast and fibroblast derived small extracellular vesicles differentially affect myoblast migration dynamics.Journal of muscle research and cell motility · 2025Article
- Role of the tendon circadian clock in tendinopathy and implications for therapeutics.International journal of experimental pathology · 2025Review
- Disruption of day-to-night changes in circadian gene expression with chronic tendinopathy.The Journal of physiology · 2024Article
- Tridimensional cell culture of dermal fibroblasts promotes exosome-mediated secretion of extracellular matrix proteins.Scientific reports · 2022Article
- Detailed Characterization of Small Extracellular Vesicles from Different Cell Types Based on Tetraspanin Composition by ExoView R100 Platform.International journal of molecular sciences · 2022Article
- Circadian regulation of protein cargo in extracellular vesicles.Science advances · 2022Article
- MCMV Centrifugal Enhancement: A New Spin on an Old Topic.Pathogens (Basel, Switzerland) · 2021Article
- Tendon and multiomics: advantages, advances, and opportunities.NPJ Regenerative medicine · 2021Review
- The Role of Exosome and the ESCRT Pathway on Enveloped Virus Infection.International journal of molecular sciences · 2021Review
- Extracellular vesicles: Critical players during cell migration.Developmental cell · 2021Review
- Understanding extracellular vesicle and nanoparticle heterogeneity: Novel methods and considerations.Proteomics · 2021Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFibroblasts are the powerhouses responsible for the production and assembly of extracellular matrix (ECM). Their activity needs to be tightly controlled especially within the musculoskeletal system, where changes to ECM composition affect force transmission and mechanical loading that are required for effective movement of the body. Extracellular vesicles (EVs) are a mode of cell-cell communication within and between tissues, which has been largely characterised in cancer. However, it is unclear what the role of healthy fibroblast-derived EVs is during tissue homeostasis.
methodsHere, we performed proteomic analysis of small EVs derived from primary human muscle and tendon cells to identify the potential functions of healthy fibroblast-derived EVs.
resultsMass spectrometry-based proteomics revealed comprehensive profiles for small EVs released from healthy human fibroblasts from different tissues. We found that fibroblast-derived EVs were more similar than EVs from differentiating myoblasts, but there were significant differences between tendon fibroblast and muscle fibroblast EVs. Small EVs from tendon fibroblasts contained higher levels of proteins that support ECM synthesis, including TGFβ1, and muscle fibroblast EVs contained proteins that support myofiber function and components of the skeletal muscle matrix.
conclusionsOur data demonstrates a marked heterogeneity among healthy fibroblast-derived EVs, indicating shared tasks between EVs of skeletal muscle myoblasts and fibroblasts, whereas tendon fibroblast EVs could play a fibrotic role in human tendon tissue. These findings suggest an important role for EVs in tissue homeostasis of both tendon and skeletal muscle in humans. Video abstract.
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