Evidence map›Paper›PMID 33147468›Full record

ArticleCell reports2020

AMPA Receptor Surface Expression Is Regulated by S-Nitrosylation of Thorase and Transnitrosylation of NSF.

George K E Umanah, Mehdi Ghasemi, Xiling Yin, Melissa Chang, Jin Wan Kim, Jianmin Zhang, Erica Ma, Leslie A Scarffe, Yun-Il Lee, Rong Chen and 11 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Redox modulation of the complement cascade contributes to synapse loss in Alzheimer's disease.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2025
    Review
  3. Cryo-EM structure of AAA + ATPase thorase reveals novel helical filament formation.Computational and structural biotechnology journal · 2025
    Article
  4. Review
  5. Review
  6. Molecular Mechanisms of AMPA Receptor Trafficking in the Nervous System.International journal of molecular sciences · 2023
    Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. Nitrosative stress in Parkinson's disease.NPJ Parkinson's disease · 2022
    Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 10 institutions in 4 countries.

George K E UmanahNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: gumanah1@jhmi.edu.
Mehdi GhasemiDepartment of Neurology, University of Massachusetts School of Medicine, Worcester, MA 01655, USA.
Xiling YinNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Melissa ChangUniversity of California, Irvine, School of Medicine, Irvine, CA 92697-3950, USA.
Jin Wan KimUniversity of Texas Health Science Center at Houston, Houston, TX 77030, USA.
Jianmin ZhangDepartment of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Beijing 100005, China.
Erica MaJohns Hopkins University Krieger School of Arts and Sciences, Baltimore, MD 21205, USA.
Leslie A ScarffeDivision of Neurology, University of British Columbia, Vancouver, BC V5Z 1M9, Canada.
Yun-Il LeeDivision of Biotechnology, Well Aging Research Center, DGIST, Daegu, Republic of Korea.
Rong ChenNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Kavya TangellaNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Amy McNamaraNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Leire Abalde-AtristainVollum Institute, Oregon Health and Science University, Portland, OR 97239, USA.
Mohamad A DarNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Samuel BennettNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Marisol CortesNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Shaida A AndrabiDepartment of Pharmacology and Toxicology, The University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Paschalis-Thomas DouliasDepartment of Pediatrics, Children's Hospital of Philadelphia Research Institute, The University of Pennsylvania, Philadelphia, PA 19104, USA.
Harry IschiropoulosDepartment of Pediatrics, Children's Hospital of Philadelphia Research Institute, The University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Pharmacology, The University of Pennsylvania, Philadelphia, PA 19104, USA.
Ted M DawsonNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Neurology, University of Massachusetts School of Medicine, Worcester, MA 01655, USA; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: tdawson@jhmi.edu.
Valina L DawsonNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Departments of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Neurology, University of Massachusetts School of Medicine, Worcester, MA 01655, USA; Graduate Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: vdawson@jhmi.edu.
Johns Hopkins University · USChildren's Hospital of Philadelphia · USChinese Academy of Medical Sciences & Peking Union Medical College · CNDaegu Gyeongbuk Institute of Science and Technology · KROregon Health & Science University · USThe University of Texas Health Science Center at Houston · USUniversity of Alabama at Birmingham · USUniversity of British Columbia · CAUniversity of California, Irvine · USUniversity of Massachusetts Chan Medical School · US

Funding

UNDERSTANDING NO SIGNALING RESULTING IN NEUROPROTECTION.P50DA000266 · NIDA · JOHNS HOPKINS UNIVERSITY · PI BARABAN, JAY M · 1985 to 2017
$45.2M
Understanding Mechanisms of a-synuclein pathologyP50NS038377 · NINDS · JOHNS HOPKINS UNIVERSITY · PI DAWSON, TED M. · 1998 to 2019
$38.8M
BIOCHEMISTRY, CELLULAR AND MOLECULAR BIOLOGY PROGRAMT32GM007445 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI GREEN, RACHEL · 1985 to 2021
$24.1M
Thorase Regulation of the Actions of CocaineP50DA044123 · NIDA · JOHNS HOPKINS UNIVERSITY · PI DAWSON, VALINA L., JANAK, PATRICIA H. · 2018 to 2022
$9.0M
Determination of the mechanism underlying Thorase modulation of AMPARs mediated neurotransmissionK01NS099362 · NINDS · JOHNS HOPKINS UNIVERSITY · PI UMANAH, GEORGE KWABENA ESSIEN · 2017 to 2019
$689k
NIDA NIH HHS P50 DA000266NIDA NIH HHS P50 DA044123NIGMS NIH HHS T32 GM007445NINDS NIH HHS K01 NS099362NINDS NIH HHS P50 NS038377
6 · The paper itself

Abstract

The regulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking affects multiple brain functions, such as learning and memory. We have previously shown that Thorase plays an important role in the internalization of AMPARs from the synaptic membrane. Here, we show that N-methyl-d-aspartate receptor (NMDAR) activation leads to increased S-nitrosylation of Thorase and N-ethylmaleimide-sensitive factor (NSF). S-nitrosylation of Thorase stabilizes Thorase-AMPAR complexes and enhances the internalization of AMPAR and interaction with protein-interacting C kinase 1 (PICK1). S-nitrosylated NSF is dependent on the S-nitrosylation of Thorase via trans-nitrosylation, which modulates the surface insertion of AMPARs. In the presence of the S-nitrosylation-deficient C137L Thorase mutant, AMPAR trafficking, long-term potentiation, and long-term depression are impaired. Overall, our data suggest that both S-nitrosylation and interactions of Thorase and NSF/PICK1 are required to modulate AMPAR-mediated synaptic plasticity. This study provides critical information that elucidates the mechanism underlying Thorase and NSF-mediated trafficking of AMPAR complexes.

Indexed as

Adenosine TriphosphatasesAmino Acid SequenceAnimalsATPases Associated with Diverse Cellular ActivitiesCell Cycle ProteinsCell MembraneCysteineEndocytosisGlutathioneHEK293 CellsHumansMice, Inbred C57BLMice, KnockoutN-Ethylmaleimide-Sensitive ProteinsNeuronal PlasticityNitric OxideAdenosine TriphosphatasesATAD1 protein, mouseATPases Associated with Diverse Cellular ActivitiesCell Cycle ProteinsCysteineGlutathioneN-Ethylmaleimide-Sensitive ProteinsNitric OxideN-MethylaspartatePrkcabp protein, mouseReceptors, AMPAS-NitrosoglutathioneAMPARATAD1endocytosisexocytosisGluA2N-ethylmaleimide-sensitive factorNMDARN-methyl-d-aspartate receptorNSFPICK1protein-interacting C kinase 1S-nitrosylationthoraseα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor

Identifiers

PMID33147468
PMCPMC7737632
OpenAlexW3097949582

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.